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Cited by 679Iron Metabolism and DisordersMetal-Catalyzed Oxygenation MechanismsTrace Elements in Health

The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.

The possible significance of the ferrous oxidase activity of ceruloplasmin in normal human serum.

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Osaki, Shigemasa, Johnson, Donald A., Frieden, Earl·1966·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)96527-0
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Abstract

The oxidation of Fe(II) by serum was studied at pH 7.35 and at various oxygen concentrations which approach the physiological conditions of human serum. The nonenzymic oxidation of Fe(II) was estimated to be insufficient to account for a rate of Fe(III)-transferrin formation necessary to provide an adequate iron supply for hemoglobin and other biosyntheses if Fe(II) is a relevant source of serum iron. The results suggested that an appreciable catalytic activity was involved in Fe(II) oxidation in serum. The ferroxidase activity of various normal human sera correlates precisely with the p-phenylenediamine oxidase activity of these sera. This catalytic activity is inhibited by azide and cyanide, and is low in sera with reduced ceruloplasmin levels. Fe(II) oxidation is also associated with the ceruloplasmin fraction on diethylaminoethyl cellulose chromatography of serum. The oxidation of Fe(II) by ceruloplasmin is zero order with respect to oxygen (10 to 200 µm), whereas the nonenzymic oxidation is first order. A biological role for the ceruloplasmin of serum in promoting the rate of iron saturation of transferrin and in stimulating iron utilization and the designation of the enzyme as serum ferroxidase is proposed.

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Crossref · 10.1016/s0021-9258(18)96527-0

Cites

20 of 20 references verified0 citation errors · 0 not checkable

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Citations

Cites

Research works printed but not linked: 3

  1. Osaki, S., McDermott, J. A., Johnson, D. A., and Frieden, E., in J. Peisach, W. Blumberg, and P. Aisen (Editors), International Symposium on the Biochemistry of Copper, Academic Press, Inc., New York, in press.
  2. Trader, 1965, Federation Proc., 24, 222
  3. Broman, 1964, Acta Soc. Med. Upsalien., 69, 75

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Metadata

Authors
Osaki, S., Johnson, D. A., & Frieden, E.
Affiliations
Florida State University (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1966-06
Volume
241
Issue
12
Pages
2746-2751
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(18)96527-0
OpenAlex
W1508143080
Cited by
679
References
20
Citations by year
2026: 2 · 2025: 5 · 2024: 8 · 2023: 10 · 2022: 10 · 2021: 18 · 2020: 7 · 2019: 12 · 2018: 15 · 2017: 9 · 2016: 15 · 2015: 17 · 2014: 21 · 2013: 16 · 2012: 17
FWCI
8.81
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
  • Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
Keywords
ceruloplasmin, ferroxidase activity, iron(II) oxidation, serum iron, oxygen dependence, iron metabolism
MeSH terms
Aniline Compounds, Humans, Iron, Oxidoreductases, Spectrophotometry, Transferrin, In Vitro Techniques

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