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Specificity of the S1 nuclease from Aspergillus oryzae.
Open accessAbstract
Conditions are described for digesting single-stranded DNA by S1 nuclease without introducing breaks in double-stranded DNA. The enzyme is inhibited by low concentrations of various compounds of phosphate. Under certain conditions S1 nuclease cleaves the strand opposite a nick in bacteriophage T5 DNA; under other conditions, the enzyme cleaves a loop in one strand of heteroduplex lambdaDNA while leaving the opposite strand intact. S1 nuclease makes many single strand breaks in ultraviolet-irradiated duplex lambdaDNA. Superhelical DNA of phiX174 (Form I) is converted first to a relaxed circular molecule (Form II), and then to a linear molecule (Form III) by cleavage at one site per molecule. Since the cleavage occurs at many sites in the population of molecules, the partially single-stranded regions in phiX174 superhelical DNA are not determined by specific nucleotide sequences.
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Cited by
Weaver, R. F., & Weissmann, C. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.5.1175 · Cited by 1,251
Wickens, M. P., Buell, G. N., & Schimke, R. T. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38098-5 · Cited by 393
Ruhanen, H., Ushakov, K., & Yasukawa, T. (2011) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2011.08.008 · Cited by 41
Metadata
- Authors
- Wiegand, R., Godson, G., & Radding, C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1975-11
- Volume
- 250
- Issue
- 22
- Pages
- 8848-8855
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1607004775
- Cited by
- 199
- References
- 0
- FWCI
- 6.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Wiegand, R., Godson, G., & Radding, C. (1975). Specificity of the S1 nuclease from Aspergillus oryzae. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)40751-5
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