The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
Open accessAbstract
Sequential reverse transcriptase, DNA polymerase, and S1 nuclease reactions can be employed to synthesize double-stranded DNA representing messenger RNA. Using reverse transcriptase products made from partially purified lysozyme, ovomucoid, and ovalbumin messengers from hen oviduct, we have characterized the Escherichia coli DNA polymerase I reaction. We have optimized for a high yield of full length second strands under conditions which require only a small amount of mRNA. The effects of several parameters (time, enzyme levels, salt concentration, monovalent cation, and temperature) on the length of products synthesized by DNA polymerase I have been investigated. Each has a significant influence on the proportion of products which are full length. Under our conditions the three reactions are efficient in synthesizing full length duplex DNA from partially purified mRNA fractions or from total poly(A)-containing RNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis
Bailey, J. M., & Davidson, N. (1976) · Analytical Biochemistry · doi:10.1016/s0003-2697(76)80049-8 · Cited by 1,298
Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
Ullrich, A., Shine, J., Chirgwin, J., Pictet, R., Tischer, E., Rutter, W. J., & Goodman, H. M. (1977) · Science · doi:10.1126/science.325648 · Cited by 1,238
Maniatis, T., Jeffrey, A., & Van deSande, H. (1975) · Biochemistry · doi:10.1021/bi00688a010 · Cited by 1,121
[29] Analysis of repeating DNA sequences by reassociation
Britten, R. J., Graham, D. E., & Neufeld, B. R. (1974) · Methods in Enzymology · doi:10.1016/0076-6879(74)29033-5 · Cited by 1,033
Enzymatic Synthesis of Deoxyribonucleic Acid
Jovin, T. M., Englund, P. T., & Bertsch, L. L. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91722-9 · Cited by 543
Enzymatic in vitro synthesis of globin genes
Efstratiadis, A., Kafatos, F. C., Maxam, A. M., & Maniatis, T. (1976) · Cell · doi:10.1016/0092-8674(76)90027-1 · Cited by 342
Synthesis of full length cDNAs from four partially purified oviduct mRNAs.
Buell, G. N., Wickens, M., Payvar, F., & SCHIMKE, R. T. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38097-3 · Cited by 270
Self-citationFull length and discrete partial reverse transcripts of globin and chorion mRNAs
Efstratiadis, A., Maniatis, T., Kafatos, F. C., Jeffrey, A., & Vournakis, J. N. (1975) · Cell · doi:10.1016/0092-8674(75)90157-9 · Cited by 218
Specificity of the S1 nuclease from Aspergillus oryzae.
Wiegand, R., Godson, G., & Radding, C. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40751-5 · Cited by 199
The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA
Monahan, J. J., Harris, S. E., Woo, S. L. C., Robberson, D. L., & O’Malley, B. W. (1976) · Biochemistry · doi:10.1021/bi00646a034 · Cited by 146
Cited by
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Pennica, D., Holmes, W. E., Kohr, W. J., Harkins, R. N., Vehar, G. A., Ward, C. A., Bennett, W. F., Yelverton, E., Seeburg, P. H., Heyneker, H. L., et al. (1983) · Nature · doi:10.1038/301214a0 · Cited by 1,243
Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin
Noda, M., Furutani, Y., Takahashi, H., Toyosato, M., Hirose, T., Inayama, S., Nakanishi, S., & Numa, S. (1982) · Nature · doi:10.1038/295202a0 · Cited by 934
Comparative biochemical properties of normal and activated human ras p21 protein
McGrath, J. P., Capon, D. J., Goeddel, D. V., & Levinson, A. D. (1984) · Nature · doi:10.1038/310644a0 · Cited by 699
Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity
Gray, P. W., Aggarwal, B. B., Benton, C. V., Bringman, T. S., Henzel, W. J., Jarrett, J. A., Leung, D. W., Moffat, B., Ng, P., Svedersky, L. P., et al. (1984) · Nature · doi:10.1038/312721a0 · Cited by 640
Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells.
Lau, L. F., & Nathans, D. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb04057.x · Cited by 567
Metadata
- Authors
- Wickens, M. P., Buell, G. N., & Schimke, R. T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1978-04
- Volume
- 253
- Issue
- 7
- Pages
- 2483-2495
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1556051370
- Cited by
- 393
- References
- 15
- FWCI
- 12.90
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. https://app.cochat.ai/papers/10.1016/s0021-9258(17)38098-5
- The paper
- Wickens, M. P., Buell, G. N., & Schimke, R. T. (1978). Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)38098-5
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
