The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
The size and detergent binding of membrane proteins.
Open accessAbstract
Sucrose density gradient centrifugation has been used to measure the binding of Triton X-100 above its critical micellar concentration to a variety of purified membrane and non-membrane proteins. In addition, binding studies were done on the three proteins below the critical micellar concentration of detergent to distinguish between the interaction of proteins with detergent monomers and detergent micelles. A procedure is described for the calculation of the molecular weight of these Triton X-100 protein complexes and measurements were made for opsin, plasma low density lipoprotein, the (Na-+ plus K-+)-dependent adenosine triphosphatase, the human red blood cell major sialoglycoprotein (PAS-1) and the human red blood cell minor glycoprotein (bandIII). These proteins behave as monomers or dimers in detergent and bind between 0.28 and 1.12 g of detergent per g of protein. A general method is also present for calculating the molecular size and shape of impure membrane proteins in detergent. Finally, Triton X-100 was shown to replace bound Na dodecyl-SO4 on the minor glycoprotein of the red blood cell.
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Cites
Automatic Recording Apparatus for Use in Chromatography of Amino Acids
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane
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A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Martin, R. G., & Ames, B. N. (1961) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64180-8 · Cited by 5,565
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
Dodge, J. T., Mitchell, C., & Hanahan, D. J. (1963) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(63)90042-0 · Cited by 4,393
[1] Measurement of molecular weights by electrophoresis on SDS-acrylamide gel
Weber, K., Pringle, J. R., & Osborn, M. (1972) · Methods in Enzymology · doi:10.1016/s0076-6879(72)26003-7 · Cited by 1,723
ENZYMATIC PROPERTIES OF THE INNER AND OUTER MEMBRANES OF RAT LIVER MITOCHONDRIA
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Purification and Properties of an Adenosine Triphosphatase from Sarcoplasmic Reticulum
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Cited by
Characterization of membrane proteins in detergent solutions
Tanford, C., & Reynolds, J. A. (1976) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(76)90009-5 · Cited by 854
Skehel, J. J., Bayley, P. M., Brown, E. B., Martin, S. R., Waterfield, M. D., White, J. M., Wilson, I. A., & Wiley, D. C. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.4.968 · Cited by 726
Ioav Cabantchik, Z., Knauf, P. A., & Rothstein, A. (1978) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(78)90016-3 · Cited by 721
Parkos, C. A., Allen, R. A., Cochrane, C. G., & Jesaitis, A. J. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113128 · Cited by 455
Topography and subunit stoichiometry of the coated vesicle proton pump.
Arai, H., Terres, G., Pink, S., & Forgac, M. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68376-0 · Cited by 276
Metadata
- Authors
- Clarke, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1975-07
- Volume
- 250
- Issue
- 14
- Pages
- 5459-5469
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1608638180
- Cited by
- 451
- References
- 29
- FWCI
- 14.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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