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Cited by 591

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Purification of a fibroblast growth factor from bovine pituitary.

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Gospodarowicz, D·1975·Journal of Biological Chemistry

Abstract

The purification from bovine pituitary gland of a growth factor responsible for the control of animal cell division in tissue culture is reported. This growth factor is a polypeptide of 13,300 molecular weight and is homogeneous when analyzed by polyacrylamide gel electrophoresis, carboxymethyl-Sephadex gradient elution chromatography, and Sephadex G-50 chromatography. The yield of growth factor is 5 mg per kg of pituitary. It is active in stimulating DNA synthesis in 3T3 cells at concentrations as low as 2 times 10-13 M with saturation at 1 times 10-10 M.

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Metadata

Authors
Gospodarowicz, D.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1975-04
Volume
250
Issue
7
Pages
2515-2520
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1490516771
Cited by
591
References
0
FWCI
13.07
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Purification of a fibroblast growth factor from bovine pituitary. https://app.cochat.ai/papers/10.1016/s0021-9258(19)41631-1
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Gospodarowicz, D. (1975). Purification of a fibroblast growth factor from bovine pituitary. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)41631-1

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.