The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Purification and Properties of C55-Isoprenoid Alcohol Phosphokinase from Staphylococcus aureus
Open accessAbstract
C55-Isoprenoid alcohol phosphokinase from Staphylococcus aureus has been purified by the following methods: cooling of a butanol extract to 0° and then to -20° followed by treatment of the dried enzyme with methanol containing increasing amounts of butanol-1. At a concentration of 30% butanol in methanol, enzyme is eluted as a lipoprotein that is purified 627-fold with respect to protein and about 40-fold with respect to lipid phosphate. Qualitative changes in the lipid composition of the purified preparation were demonstrated as well as a simplification of the protein distribution seen on sodium dodecyl sulfate gels. Some properties of this lipoprotein are described. Two more purification steps in organic solvents (chromatography on DEAE-cellulose and chromatography on hydroxypropylated Sephadex G-50) led to apoprotein of near homogeneity. The hydroxypropylated Sephadex G-50 column was shown to separate proteins below molecular weight of 30,000 according to molecular weight. The enzyme activity appeared to be associated with a single polypeptide chain of molecular weight 17,000.
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Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Cited by
Sinensky, M. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.2.522 · Cited by 1,517
Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins
Hancock, R. E., & Carey, A. M. (1979) · Journal of Bacteriology · doi:10.1128/jb.140.3.902-910.1979 · Cited by 425
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus
Ton-That, H., Mazmanian, S. K., Faull, K. F., & Schneewind, O. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.13.9876 · Cited by 359
Macfarlane, E. L. A., Kwasnicka, A., Ochs, M. M., & Hancock, R. E. W. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01600.x · Cited by 254
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus
Perry, A. M., Ton-That, H., Mazmanian, S. K., & Schneewind, O. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m109194200 · Cited by 218
Metadata
- Authors
- Sandermann, H., & Strominger, J. L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1972-08
- Volume
- 247
- Issue
- 16
- Pages
- 5123-5131
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1520097912
- Cited by
- 285
- References
- 1
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sandermann, H., & Strominger, J. L. (1972). Purification and Properties of C55-Isoprenoid Alcohol Phosphokinase from Staphylococcus aureus. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)44948-x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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