The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins
Open accessAbstract
A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was determined by the solubilization temperature. Four of these heat-modifiable proteins had characteristics similar to protein II of the Escherichia coli outer membrane. Addition of lipopolysaccharide subsequent to solubilization caused reversal of the heat modification. The other heat-modifiable protein, the porin protein F, was unusually stable to sodium dodecyl sulfate. Long periods of boiling in sodium dodecyl sulfate were required to cause conversion to the heat-modified form. This was demonstrated both with outer membrane-associated and purified lipopolysaccharide-depleted protein F. Furthermore, lipopolysaccharide treatment had no effect on the mobility of heat-modified protein F. Thus it is concluded that protein F represents a new class of heat-modifiable protein. It was further demonstrated that the electrophoretic mobility of protein F was modified by 2-mercaptoethanol and that the 2-mercaptoethanol and heat modification of mobility were independent of one another. The optimal conditions for the examination of the outer membrane proteins of P. aeruginosa by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis are discussed.
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Cites
Neville, D. M. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61792-2 · Cited by 1,989
A simplified method for the quantitative assay of small amounts of protein in biologic material
Schacterle, G. R., & Pollack, R. L. (1973) · Analytical Biochemistry · doi:10.1016/0003-2697(73)90523-x · Cited by 1,794
Mechanism of Assembly of the Outer Membrane of Salmonella typhimurium
Osborn, M. J., Gander, J. E., Parisi, E., & Carson, J. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45127-2 · Cited by 1,568
Lugtenberg, B., Meijers, J., Peters, R., van der Hoek, P., & van Alphen, L. (1975) · FEBS Letters · doi:10.1016/0014-5793(75)80272-9 · Cited by 1,416
The Outer Membrane Proteins of Gram-Negative Bacteria: Biosynthesis, Assembly, and Functions
DiRienzo, J. M., Nakamura, K., & Inouye, M. (1978) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.47.070178.002405 · Cited by 455
Ames, G. F.-L., Spudich, E. N., & Nikaido, H. (1974) · Journal of Bacteriology · doi:10.1128/jb.117.2.406-416.1974 · Cited by 332
Purification and Properties of C55-Isoprenoid Alcohol Phosphokinase from Staphylococcus aureus
Sandermann, H., & Strominger, J. L. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)44948-x · Cited by 285
NAKAMURA, K., & MIZUSHIMA, S. (1976) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a131414 · Cited by 264
de Jong, W. W., Zweers, A., & Cohen, L. H. (1978) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(78)90907-5 · Cited by 235
Schweizer, M., HINDENNACH, I., Garten, W., & Henning, U. (1978) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1978.tb12013.x · Cited by 218
Cited by
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
Nikaido, H. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.593-656.2003 · Cited by 4,316
Lugtenberg, B., & Van Alphen, L. (1983) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(83)90014-x · Cited by 759
Structure, function and regulation of Pseudomonas aeruginosa porins
Chevalier, S., Bouffartigues, E., Bodilis, J., Maillot, O., Lesouhaitier, O., Feuilloley, M. G. J., Orange, N., Dufour, A., & Cornelis, P. (2017) · FEMS Microbiology Reviews · doi:10.1093/femsre/fux020 · Cited by 423
Trias, J., & Nikaido, H. (1990) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.34.1.52 · Cited by 291
Livermore, D. M. (1992) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.36.9.2046 · Cited by 288
Metadata
- Authors
- Hancock, R. E., & Carey, A. M.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1979-12
- Volume
- 140
- Issue
- 3
- Pages
- 902-910
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1606263729
- Cited by
- 425
- References
- 20
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- green
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- Hancock, R. E., & Carey, A. M. (1979). Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins. Journal of Bacteriology. https://doi.org/10.1128/jb.140.3.902-910.1979
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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