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A SPECIFIC MICROMETHOD FOR THE DETERMINATION OF ACYL PHOSPHATES
Open accessAbstract
During the last few years it was observed that anhydrides of carboxylic and phosphoric acids appear as intermediates in metabolism. For one of these, acetyl phosphate, a method of determination had been worked out (I), based on a differential precipitation of calcium acetyl and inorganic phosphate with subsequent phosphate determination in the separate fractions. Such a procedure, founded on a more or less fortuitous behavior of the acetyl phosphate, lacked a desirable specificity for the characteristic anhydride group. In this communication, a method is described which utilizes the reaction of acyl phosphates with hydroxylamine. The acyl part of the acid anhydride is converted into hydroxamic acid.
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Citations
Cited by
Srere, P. A. (1969) · Methods in Enzymology · doi:10.1016/0076-6879(69)13005-0 · Cited by 2,005
Hestrin, S. (1949) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)56740-5 · Cited by 1,923
Varma, A., & Palsson, B. O. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.10.3724-3731.1994 · Cited by 1,051
Cyclopiazonic Acid is a Specific Inhibitor of the Ca2+-ATPase of Sarcoplasmic Reticulum
Seidler, N. W., Jona, I., Vegh, M., & Martonosi, A. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)84646-x · Cited by 746
Co‐metabolism of citrate and glucose by Leuconostoc spp.: effects on growth, substrates and products
Cogan, T. M. (1987) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1987.tb02726.x · Cited by 135
Metadata
- Authors
- Lipmann, F., & Tuttle, L. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1945-06
- Volume
- 159
- Issue
- 1
- Pages
- 21-28
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1547207533
- Cited by
- 1,413
- References
- 0
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lipmann, F., & Tuttle, L. C. (1945). A SPECIFIC MICROMETHOD FOR THE DETERMINATION OF ACYL PHOSPHATES. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)51298-4
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