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A homogeneous type II DNA topoisomerase from HeLa cell nuclei.
Open accessAbstract
Using kinetoplast DNA networks as a substrate in a decatenation assay, we have purified to apparent homogeneity a type II DNA topoisomerase from HeLa cell nuclei. The most pure preparations contain a single polypeptide of 172,000 daltons as determined by sodium dodecyl sulfate-gel electrophoresis. The molecular weight of the native protein, based on sedimentation and gel filtration analyses, is estimated to be 309,000. These results suggest that the enzyme is a dimer of 172,0090-dalton subunits. The enzyme is a type II topoisomerase as demonstrated by its ability to change the linking number of DNA circles in steps of two and to decatenate or unknot covalently closed DNA circles. No gyrase activity is detectable. ATP is required for the relaxation, decatenation, and unknotting of DNA, and a DNA-dependent ATPase activity is present in the most pure fractions. ATP is hydrolyzed to ADP in this properties to T4 DNA topoisomerase (Liu, L. F., Liu, C. C., and Alberts, B. M. (1979) Nature 281, 456-461).
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Cited by
Adriamycin-Induced DNA Damage Mediated by Mammalian DNA Topoisomerase II
Tewey, K. M., Rowe, T. C., Yang, L., Halligan, B. D., & Liu, L. F. (1984) · Science · doi:10.1126/science.6093249 · Cited by 1,538
Self-citationTewey, K. M., Chen, G. L., Nelson, E. M., & Liu, L. F. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)47282-6 · Cited by 887
Self-citationMechanism of action of eukaryotic topoisomerase II and drugs targeted to the enzyme
Burden, D. A., & Osheroff, N. (1998) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(98)00132-8 · Cited by 503
DNA topoisomerases: harnessing and constraining energy to govern chromosome topology
Schoeffler, A. J., & Berger, J. M. (2008) · Quarterly Reviews of Biophysics · doi:10.1017/s003358350800468x · Cited by 490
Drake, F., Zimmerman, J., McCabe, F., Bartus, H., Per, Sr., Sullivan, D., Ross, W., Mattern, M., Johnson, R., & Crooke, S. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)49317-9 · Cited by 277
Metadata
- Authors
- Miller, K. G., Liu, L. F., & Englund, P. T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1981-09
- Volume
- 256
- Issue
- 17
- Pages
- 9334-9339
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1566761847
- Cited by
- 356
- References
- 0
- FWCI
- 7.95
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Miller, K. G., Liu, L. F., & Englund, P. T. (1981). A homogeneous type II DNA topoisomerase from HeLa cell nuclei. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)52552-2
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