The DOI matches its Crossref record.
Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli.
Open accessAbstract
Beta-Ketoacyl-acyl carrier protein synthases I and II of Escherichia coli were purified and characterized. Synthase I was shown to have a molecular weight of 80,000 +/- 5,000 and to be composed of two similarly sized subunits. Synthase II had a molecular weight of 85,000 +/- 5,000 and also was apparently homodimeric. Gel electrophoresis of partial proteolytic digests demonstrated that synthases I and II share few if any common peptides. Synthases I and II also were shown to be unrelated by immunological criteria. An improved assay for beta-ketoacyl-acyl carrier protein synthase activity gave kinetic parameters for synthases I and II at both 27 degrees C and 37 degrees C using five long chain acyl-acyl carrier protein substrates. The properties of synthase II are consistent with the proposed role of this enzyme in the modulation of fatty acid synthesis by temperature. fabF mutants of E. coli lack synthase II. The fabF locus was mapped at min 24.5 of the E. coli genetic map and the clockwise map order was found to be pyrC, fabD, fabF, purB.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Linkage map of Escherichia coli K-12, edition 7
Bachmann, B. (1983) · Microbiological Reviews · doi:10.1128/mmbr.47.2.180-230.1983 · Cited by 1,438
THE STRUCTURAL BIOLOGY OF TYPE II FATTY ACID BIOSYNTHESIS
White, S. W., Zheng, J., Zhang, Y.-M., & Rock, C. O. (2005) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133524 · Cited by 832
Genetic Contributions to Understanding Polyketide Synthases
Hopwood, D. A. (1997) · Chemical Reviews · doi:10.1021/cr960034i · Cited by 710
Bacterial Fatty Acid Biosynthesis: Targets for Antibacterial Drug Discovery
Campbell, J. W., & Cronan, J. E. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.305 · Cited by 479
Self-citationEscherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesis
Rock, C. O., & Cronan, J. E. (1996) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(96)00056-2 · Cited by 332
Self-citation
Metadata
- Authors
- Garwin, J. L., Klages, A. L., & Cronan, J. E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1980-12
- Volume
- 255
- Issue
- 24
- Pages
- 11949-11956
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1699689362
- Cited by
- 207
- References
- 0
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli. https://app.cochat.ai/papers/10.1016/s0021-9258(19)70226-9
- The paper
- Garwin, J. L., Klages, A. L., & Cronan, J. E. (1980). Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)70226-9
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
