The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Release of transforming growth factor-beta 1 from the pericellular matrix of cultured fibroblasts and fibrosarcoma cells by plasmin and thrombin.
Open accessAbstract
A sensitive immunoblotting assay was developed for the detection of transforming growth factor (TGF)-beta 1 from cell extracts and culture medium. HT-1080 human fibrosarcoma cells and human fibroblasts were used as models for the secretion and proteolytic release of pericellular matrix-associated TGF-beta 1. Analysis of the pericellular matrices of the cells indicated that the majority of cell-layer associated TGF-beta 1 was associated with the pericellular matrix. Treatment of the cells with plasmin or thrombin released the matrix-associated TGF-beta 1 to the culture medium. Assays for the biological activity of plasmin-released TGF-beta 1 by Mv1Lu cell growth inhibition assays indicated that the majority was in the latent form. Northern hybridization analyses indicated that the mRNA levels of TGF-beta 1 were not elevated during the proteinase treatment. Experiments using radiolabeled TGF-beta 1 indicated that exogenous active TGF-beta 1 associates mainly with the presumed TGF-beta 1 receptors that were not retained in the extracellular matrix preparations. These results indicate that a major fraction of latent TGF-beta 1 that is produced by the cells is deposited to and remains associated with the pericellular matrices of cultured fibroblasts and fibrosarcoma cells, and that matrix-associated TGF-beta 1 is very susceptible to release by various proteolytic enzymes.
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Sato, Y., Tsuboi, R., Lyons, R., Moses, H., & Rifkin, D. B. (1990) · The Journal of cell biology · doi:10.1083/jcb.111.2.757 · Cited by 444
Andres, J. L., Stanley, K., Cheifetz, S., & Massagué, J. (1989) · The Journal of cell biology · doi:10.1083/jcb.109.6.3137 · Cited by 354
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Metadata
- Authors
- Taipale, J., Koli, K., & Keski-Oja, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-12
- Volume
- 267
- Issue
- 35
- Pages
- 25378-25384
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1529637720
- Cited by
- 201
- References
- 2
- FWCI
- 3.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Taipale, J., Koli, K., & Keski-Oja, J. (1992). Release of transforming growth factor-beta 1 from the pericellular matrix of cultured fibroblasts and fibrosarcoma cells by plasmin and thrombin. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)74051-4
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