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The effect of GTP and Mg2+ on the GTPase activity and the fluorescent properties of Go.
Open accessAbstract
The structures of the guanosine 5'O-(3-thio)triphosphate (GTP gamma S)-containing guanine nucleotide-binding regulatory proteins (G proteins) are distinct from those of the GDP-containing forms. One indication of the conformational change caused by GTP gamma S is a Mg2+-sensitive increase in the intensity of the proteins' tryptophan fluorescence (Higashijima, T., Ferguson, K.M., Sternweis, P.C., Ross, E.M., Smigel, M.D., Gilman, A.G. (1987), J. Biol. Chem., 262, 762-766). GTP causes a similar change in the fluorescence of Go, a G protein from bovine brain. When Mg2+ is also present, the increase in fluorescence is transient, and the rate of decline in the intensity of the fluorescence is the same as the rate of GTP hydrolysis by the protein. The steady-state rate of hydrolysis of GTP by Go (0.3-0.4/min) is slower than the catalytic rate of the protein (2/min), because the rate-limiting step in the reaction is the release of GDP.
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Cited by
Receptor-effector coupling by G proteins
Birnbaumer, L., Abramowitz, J., & Brown, A. M. (1990) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(90)90007-y · Cited by 1,118
Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine
Flatau, G., Lemichez, E., Gauthier, M., Chardin, P., Paris, S., Fiorentini, C., & Boquet, P. (1997) · Nature · doi:10.1038/42743 · Cited by 498
Higashijima, T., Ferguson, K. M., Sternweis, P. C., Smigel, M. D., & Gilman, A. G. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)75851-7 · Cited by 366
Self-citationKozasa, T., & Gilman, A. G. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.4.1734 · Cited by 334
Self-citationRapid GDP release from Gsα in patients with gain and loss of endocrine function
Iiri, T., Herzmark, P., Nakamoto, J. M., Van Dop, C., & Bourne, H. R. (1994) · Nature · doi:10.1038/371164a0 · Cited by 295
Metadata
- Authors
- Higashijima, T., Ferguson, K. M., Smigel, M. D., & Gilman, A. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1987-01
- Volume
- 262
- Issue
- 2
- Pages
- 757-761
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1560656625
- Cited by
- 232
- References
- 0
- FWCI
- 4.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Higashijima, T., Ferguson, K. M., Smigel, M. D., & Gilman, A. G. (1987). The effect of GTP and Mg2+ on the GTPase activity and the fluorescent properties of Go. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)75850-5
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