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Cited by 366

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins.

Open access
Higashijima, T, Ferguson, K M, Sternweis, P C, Smigel, M D, Gilman, A G·1987·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(19)75851-7
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Abstract

Mg2+ interacts with the alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) in the presence of guanosine-5'-[gamma-thio]triphosphate (GTP-gamma S) to form a highly fluorescent complex from which nucleotide dissociates very slowly. The apparent Kd for interaction of G alpha X GTP gamma S with Mg2+ is approximately 5 nM, similar to the Km for G protein GTPase activity X G beta gamma increases the rate of dissociation of GTP gamma S from G alpha X GTP gamma S or G alpha X GTP gamma S X Mg2+ at low concentrations of Mg2+. When the concentration of Mg2+ exceeds 1 mM, G beta gamma dissociates from G beta gamma X G alpha X GTP gamma S X Mg2+. Compared with the dramatic effect of Mg2+ on binding of GTP gamma S to G alpha, the metal has relatively little effect on the binding of GDP. However, G beta gamma increases the affinity of G alpha for GDP by more than 100-fold. High concentrations of Mg2+ promote the dissociation of GDP from G beta gamma X G alpha X GDP, apparently without causing subunit dissociation. The steady-state rate of GTP hydrolysis is strictly correlated with the rate of dissociation of GDP from G alpha under all conditions examined. Thus, there are at least two sites for interaction of Mg2+ with G protein-nucleotide complexes. Furthermore, binding of G beta gamma and GTP gamma S to G alpha is negatively cooperative, while the binding interaction between G beta gamma and GDP is strongly positive.

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Metadata

Authors
Higashijima, T., Ferguson, K. M., Sternweis, P. C., Smigel, M. D., & Gilman, A. G.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1987-01
Volume
262
Issue
2
Pages
762-766
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(19)75851-7
OpenAlex
W1499695147
Cited by
366
References
11
FWCI
7.40
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins. https://app.cochat.ai/papers/10.1016/s0021-9258(19)75851-7
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Higashijima, T., Ferguson, K. M., Sternweis, P. C., Smigel, M. D., & Gilman, A. G. (1987). Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)75851-7

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.