Cited by 151Genomics and Chromatin DynamicsGlutathione Transferases and PolymorphismsGenomics, phytochemicals, and oxidative stress

Structural and functional analysis of an enhancer GPEI having a phorbol 12-O-tetradecanoate 13-acetate responsive element-like sequence found in the rat glutathione transferase P gene

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Okuda, A, Imagawa, M, Maeda, Y, Sakai, M, Muramatsu, M·1989·Journal of Biological Chemistry

Abstract

We have recently identified a typical enhancer, termed GPEI, located about 2.5 kilobases upstream from the transcription initiation site of the rat glutathione transferase P gene. Analyses of 5' and 3' deletion mutants revealed that the cis-acting sequence of GPEI contained the phorbol 12-O-tetradecanoate 13-acetate responsive element (TRE)-like sequence in it. For the maximal activity, however, GPEI required an adjacent upstream sequence of about 19 base pairs in addition to the TRE-like sequence. With the DNA binding gel-shift assay, we could detect protein(s) that specifically binds to the TRE-like sequence of GPEI fragment, which was possibly c-jun.c-fos complex or a similar protein complex. The sequence immediately upstream of the TRE-like sequence did not have any activity by itself, but augmented the latter activity by about 5-fold.

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Crossref · 10.1016/s0021-9258(19)84795-6

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    “when ferrous iron interacts with H2O2, it undergoes the Fenton reaction (229). The Fenton reaction produces ferric iron, −OH, and the hydroxyl radical. It may also result in the peroxidation of adjacent lipids and lead to oxidative damage of DNA and other macromolecules.”
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Authors
Okuda, A., Imagawa, M., Maeda, Y., Sakai, M., & Muramatsu, M.
Affiliations
Hokkaido University (JP)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1989-10
Volume
264
Issue
28
Pages
16919-16926
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1581676497
Cited by
151
References
31
Citations by year
2022: 1 · 2021: 1 · 2018: 1 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 2
FWCI
2.70
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
Keywords
cis-acting enhancer, transcriptional regulation, protein-DNA interactions, rat gene
MeSH terms
Animals, Base Sequence, Chromosome Deletion, Enhancer Elements, Genetic, Genes, Glutathione Transferase, Molecular Sequence Data, Mutation, Plasmids, Regulatory Sequences, Nucleic Acid, Tetradecanoylphorbol Acetate, Transcription, Genetic, Restriction Mapping, Oligonucleotide Probes, Rats

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