The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Heat shock-induced translational alterations in HeLa cells. Initiation factor modifications and the inhibition of translation.
Open accessAbstract
Heat shock at 45 degrees C virtually abolishes protein synthesis in HeLa cells, but return to 37 degrees C effects a complete recovery and the concomitant synthesis of heat shock-induced proteins. Heat shock induces polysome disaggregation, indicating initiation is principally inhibited. In vitro assays for initiation factor activities reveal heat shock inhibits eukaryotic initiation factor 2 (eIF-2), eIF-(3 + 4F), and eIF-4B. Immunoblot analyses show that eIF-2 alpha and eIF-2 beta become modified during heat shock, and eIF-4B variants disappear. Upon return to 37 degrees C, these alterations reverse. The modifications of eIF-2 alpha and eIF-4B are due to phosphorylation and dephosphorylation, respectively. Enzymatic activities induced by heat shock inhibit protein synthesis and modify initiation factors in a rabbit reticulocyte lysate. Initiation factor modifications may contribute to, or cause, protein synthesis inhibition.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates
PELHAM, H. R. B., & JACKSON, R. J. (1976) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1976.tb10656.x · Cited by 3,926
Etchison, D., Milburn, S. C., Edery, I., Sonenberg, N., & Hershey, J. W. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33352-0 · Cited by 583
Self-citationThe mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.
Benne, R., & Hershey, J. W. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40805-2 · Cited by 433
Self-citationNew initiation factor activity required for globin mRNA translation.
Grifo, J. A., Tahara, S. M., Morgan, M. A., Shatkin, A. J., & Merrick, W. C. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)81965-6 · Cited by 406
Purification of the major mammalian heat shock proteins.
Welch, W. J., & Feramisco, J. R. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33376-3 · Cited by 362
Initiation of mammalian protein synthesis
Trachsel, H., Erni, B., Schreier, M. H., & Staehelin, T. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90269-8 · Cited by 342
Regulation of protein synthesis in HeLa cells: Translation at elevated temperatures
McCormick, W., & Penman, S. (1969) · Journal of Molecular Biology · doi:10.1016/0022-2836(69)90320-9 · Cited by 296
The Regulation of Initiation of Mammalian Protein Synthesis
Jagus, R., Anderson, W. F., & Safer, B. (1981) · Progress in Nucleic Acid Research and Molecular Biology · doi:10.1016/s0079-6603(08)60484-5 · Cited by 252
Regulation of protein synthesis initiation in eucaryotes
Ochoa, S. (1983) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(83)90598-2 · Cited by 222
Identification of the hypusine-containing protein hy+ as translation initiation factor eIF-4D.
Cooper, H. L., Park, M. H., Folk, J. E., Safer, B., & Braverman, R. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.7.1854 · Cited by 213
Cited by
Brain ischemia and reperfusion: molecular mechanisms of neuronal injury
White, B. C., Sullivan, J. M., DeGracia, D. J., O’Neil, B. J., Neumar, R. W., Grossman, L. I., Rafols, J. A., & Krause, G. S. (2000) · Journal of the Neurological Sciences · doi:10.1016/s0022-510x(00)00386-5 · Cited by 796
Mazroui, R. (2002) · Human Molecular Genetics · doi:10.1093/hmg/11.24.3007 · Cited by 369
Widespread Regulation of Translation by Elongation Pausing in Heat Shock
Shalgi, R., Hurt, J. A., Krykbaeva, I., Taipale, M., Lindquist, S., & Burge, C. B. (2013) · Molecular Cell · doi:10.1016/j.molcel.2012.11.028 · Cited by 339
Gallie, D. R. (1998) · Gene · doi:10.1016/s0378-1119(98)00318-7 · Cited by 280
Distinctive Properties of the 5′-Untranslated Region of Human Hsp70 mRNA
Rubtsova, M. P., Sizova, D. V., Dmitriev, S. E., Ivanov, D. S., Prassolov, V. S., & Shatsky, I. N. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m303213200 · Cited by 100
Metadata
- Authors
- Duncan, R., & Hershey, J. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-10
- Volume
- 259
- Issue
- 19
- Pages
- 11882-11889
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1506260722
- Cited by
- 277
- References
- 24
- FWCI
- 9.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Heat shock-induced translational alterations in HeLa cells. Initiation factor modifications and the inhibition of translation. https://app.cochat.ai/papers/10.1016/s0021-9258(20)71294-9
- The paper
- Duncan, R., & Hershey, J. W. (1984). Heat shock-induced translational alterations in HeLa cells. Initiation factor modifications and the inhibition of translation. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(20)71294-9
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
