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Cloning and expression of cDNA for a mouse EP1 subtype of prostaglandin E receptor.
Open accessAbstract
A functional cDNA clone encoding a mouse EP1 subtype of prostaglandin (PG) E receptor was isolated from a mouse cDNA library by cross-hybridization with the mouse thromboxane A2 receptor cDNA. The clone isolated encodes a protein consisting of 405 amino acid residues with putative seven-transmembrane domains. [3H]PGE2 specifically bound to the membrane of Chinese hamster ovary cells expressing this clone. The binding to the membrane was displaced with unlabeled PGs in the order of PGE2 > iloprost (a prostacyclin analogue) > PGE1 > PGF2 alpha > U-46619 (a thromboxane A2 analogue) > PGD2. The binding was also inhibited by 17-phenyl trinor PGE2 (an EP1 agonist) and sulprostone (an EP1 and EP3 agonist) but not by 11-deoxy PGE1 (an EP2 and EP3 agonist) and butaprost (an EP2 agonist). PGE2 induced a rapid increase in intracellular Ca2+ concentration in Chinese hamster ovary cells expressing the receptor. These results suggest that this receptor belongs to EP1 subtype of PGE receptor. Northern blot analysis demonstrated that the mRNA of this receptor is expressed abundantly in kidney and in a lessor amount in lung.
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Cited by
Prostanoid Receptors: Subtypes and Signaling
Breyer, R. M., Bagdassarian, C. K., Myers, S. A., & Breyer, M. D. (2001) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.41.1.661 · Cited by 1,011
Prostaglandin E2 Stimulates Aromatase Expression in Endometriosis-Derived Stromal Cells
Noble, L. S. (1997) · Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.82.2.600 · Cited by 301
Molecular cloning and characterization of the four rat prostaglandin E2 prostanoid receptor subtypes
Boie, Y., Stocco, R., Sawyer, N., Slipetz, D. M., Ungrin, M. D., Neuschäfer-Rube, F., Püschel, G. P., Metters, K. M., & Abramovitz, M. (1997) · European Journal of Pharmacology · doi:10.1016/s0014-2999(97)01383-6 · Cited by 296
Prostaglandin E 2 as a Regulator of Immunity to Pathogens
Martínez-Colón, G. J., & Moore, B. B. (2018) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2017.12.008 · Cited by 109
UV-induced squamous cell carcinoma—a role for antiapoptotic signalling pathways
Rodust, P. M., Stockfleth, E., Ulrich, C., Leverkus, M., & Eberle, J. (2009) · British Journal of Dermatology · doi:10.1111/j.1365-2133.2009.09458.x · Cited by 105
Metadata
- Authors
- Watabe, A., Sugimoto, Y., Honda, A., Irie, A., Namba, T., Negishi, M., Ito, S., Narumiya, S., & Ichikawa, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-09
- Volume
- 268
- Issue
- 27
- Pages
- 20175-20178
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1523969239
- Cited by
- 372
- References
- 0
- FWCI
- 28.35
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Watabe, A., Sugimoto, Y., Honda, A., Irie, A., Namba, T., Negishi, M., Ito, S., Narumiya, S., & Ichikawa, A. (1993). Cloning and expression of cDNA for a mouse EP1 subtype of prostaglandin E receptor. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(20)80710-8
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