The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice
Abstract
Isotyping and quantitation of murine IgG2a antibodies are widely performed with commercial monoclonal and polyclonal antisera raised against BALB/c IgG2a myeloma proteins. Recently it became evident that inbred mouse strains with the Igh1-b allele do not have the gene for IgG2a and instead express the IgG2c isotype. We show that commercial anti-IgG2a sera cross-react inadequately against IgG2c in immunoblot and ELISA and hence, are not suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD. We have used DNA immunization to generate polyclonal anti-IgG2c serum and demonstrated that it is essential to use IgG2c-specific antiserum to quantify accurately isotypic responses in mouse strains with the Igh1-b allele.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
C57BL/6 mice lack the IgG2a isotype.
“IgG2c isotype was selected for C57BL/6 sera due to their lack of IgG2a isotype (64)”
From An <i>Alphavirus</i> -derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates (2020) · cites this paper as [64]
Cites
Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells
Stevens, T. L., Bossie, A., Sanders, V. M., Fernandez-Botran, R., Coffman, R. L., Mosmann, T. R., & Vitetta, E. S. (1988) · Nature · doi:10.1038/334255a0 · Cited by 933 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Winer, D. A., Winer, S., Shen, L., Wadia, P. P., Yantha, J., Paltser, G., Tsui, H., Wu, P., Davidson, M. G., Alonso, M. N., et al. (2011) · Nature Medicine · doi:10.1038/nm.2353 · Cited by 1,050 · see citing works
Erasmus, J. H., Khandhar, A. P., O’Connor, M. A., Walls, A. C., Hemann, E. A., Murapa, P., Archer, J., Leventhal, S., Fuller, J. T., Lewis, T. B., et al. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.abc9396 · Cited by 241 · see citing works
Young, G. R., Ploquin, M. J.-Y., Eksmond, U., Wadwa, M., Stoye, J. P., & Kassiotis, G. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002709 · Cited by 56 · see citing works
- Authors
- Martin, R. M., Brady, J. L., & Lew, A. M.
- Affiliations
- The Royal Melbourne Hospital (AU)
- Corresponding author
- Lew, Andrew M.
- ORCID
- Lew, Andrew M.
- Venue
- Journal of Immunological Methods
- Publisher
- Elsevier BV
- Published
- 1998-03
- Volume
- 212
- Issue
- 2
- Pages
- 187-192
- ISSN
- 0022-1759 (ISSN-L)
- OpenAlex
- W1982895831
- Cited by
- 330
- References
- 1
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 12 · 2023: 8 · 2022: 7 · 2021: 13 · 2020: 10 · 2019: 10 · 2018: 11 · 2017: 10 · 2016: 13 · 2015: 18 · 2014: 24 · 2013: 18 · 2012: 12
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Immunological Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Diabetes Australia
- National Health and Medical Research Council
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
- Keywords
- IgG2c, IgG2a, antibody isotyping, C57BL/6 mice, NOD mice, DNA vaccine, murine antibodies
- MeSH terms
- Alleles, Animals, Antibody Specificity, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Genes, Immunoglobulin, Immunoglobulin G, Immunoassay, Immunoglobulin Isotypes, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred Strains, Immunoblotting, Reproducibility of Results, Mice, Inbred NOD, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
