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Determination of cholesterol using o-phthalaldehyde
Open accessAbstract
A simple, rapid method for the determination of cholesterol in plasma and tissue using o-phthalaldehyde is presented. Comparison of this method with the FeCl(3) method gave identical results. However, the o-phthalaldehyde determination is three times more sensitive than the FeCl(3) determination (molar extinction coefficients of 11,610 and 33,440 for FeCl(3) and o-phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation.
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Citations
Cited by
Assimilation of cholesterol by Lactobacillus acidophilus
Gilliland, S. E., Nelson, C. R., & Maxwell, C. (1985) · Applied and Environmental Microbiology · doi:10.1128/aem.49.2.377-381.1985 · Cited by 649
Plasma very low density lipoproteins contain a single molecule of apolipoprotein B
Elovson, J., Chatterton, J. E., Bell, G. T., Schumaker, V. N., Reuben, M. A., Puppione, D. L., Reeve, J. R., & Young, N. L. (1988) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38425-x · Cited by 266
Yasmin, I., Saeed, M., Khan, W. A., Khaliq, A., Chughtai, M. F. J., Iqbal, R., Tehseen, S., Naz, S., Liaqat, A., Mehmood, T., et al. (2020) · Microorganisms · doi:10.3390/microorganisms8030354 · Cited by 233
Intracellular movement of cholesterol in rat adrenal cells. Kinetics and effects of inhibitors
Crivello, J. F., & Jefcoate, C. R. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70620-6 · Cited by 230
Triglyceride-lowering effect of marine polyunsaturates in patients with hypertriglyceridemia.
Sanders, T. A., Sullivan, D. R., Reeve, J., & Thompson, G. R. (1985) · Arteriosclerosis: An Official Journal of the American Heart Association, Inc. · doi:10.1161/01.atv.5.5.459 · Cited by 212
Metadata
- Authors
- Rudel, L. L., & Morris, M. D.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1973-05
- Volume
- 14
- Issue
- 3
- Pages
- 364-366
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2104010651
- Cited by
- 1,043
- References
- 0
- FWCI
- 35.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
