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Cited by 586

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Quantitative determination of the neutral glycosyl ceramides in human blood

Vance, Dennis E., Sweeley, Charles C.·1967·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)38884-2
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Abstract

A method is described for the qualitative and quantitative estimation of four neutral glycosyl ceramides from human plasma and erythrocytes. Total lipids extracted from 50 ml of plasma or packed erythrocytes were separated by silicic acid chromatography into neutral lipids, a fraction of mixed glycolipids that was eluted with acetone-methanol 9:1, and phospholipids. After mild alkali-catalyzed methanolysis to remove contaminants from the crude fraction of glycolipids, individual glycosyl ceramides were isolated by preparative thin-layer chromatography. The oligosaccharide portions of these lipids were characterized by cleavage with methanolic hydrogen chloride and gas chromatography of the O-trimethylsilyl methyl glycosides. It was possible to study the composition of the carbohydrate and sphingolipid base fractions in the same gas chromatographic analysis. With mannitol as an internal standard for gas chromatographic estimation of glucose, concentrations of each of the glycosyl ceramides were determined with a precision of about 10%. Recoveries of the lipids from plasma varied with the complexity of the oligosaccharide moiety and ranged from 94% with glucosyl ceramide to 71% with globoside. Concentrations of the four glycosyl ceramides in plasma and in erythrocytes were determined for samples from young, healthy males. Amounts of glycolipid as low as 0.1 micromole can be determined conveniently by this procedure.

Overview

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Registry

Crossref · 10.1016/s0022-2275(20)38884-2

Cites

35 of 35 references verified0 citation errors · 0 not checkable

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Verification

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Citations

Cites

Research works printed but not linked: 5

  1. Klenk, 1951, Z. Physiol. Chem., 288, 220
  2. Yamakawa, 1965, Japan. J. Exptl. Med., 35, 201
  3. Radin, 1957, Federation Proc., 16, 825
  4. Vance, 1967, Federation Proc., 26, 277
  5. Wolfrom, 1962, 1, 121

Cited by

See all 586

Metadata

Authors
Vance, D. E., & Sweeley, C. C.
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1967-11
Volume
8
Issue
6
Pages
621-630
ISSN
0022-2275
DOI
10.1016/s0022-2275(20)38884-2
Cited by
586
References
35
Language
en
Licence

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.