The DOI matches its Crossref record.
Identification of mono- and dihydroxy bile acids in human feces by gas-liquid chromatography and mass spectrometry
Open accessAbstract
The mono- and disubstituted cholanoic acids present in human feces have been investigated. Extracts of feces were fractionated on silicic acid column and individual bile acids were isolated by preparative thin-layer chromatography. The isolated compounds were studied by gas-liquid chromatography of the methyl esters, partial trimethylsilyl ethers, oxidation products, and trifluoroacetates. The probable structures deduced were confirmed by gas chromatography-mass spectrometry and by comparisons with authentic compounds. The following derivatives of 5 Beta-cholanoic acid not previously isolated from human feces were identified: 3,12-diketo, 3-keto-12alpha-hydroxy, 3alpha,12 Beta-dihydroxy, 3 Beta,12 Beta-dihydroxy, 3-keto-7alpha-hydroxy, 3alpha-hydroxy-7-keto, 3 Beta,7alpha-dihydroxy, 3alpha,7alpha-dihydroxy, and 3alpha,7 Beta-dihydroxy. The presence of 3-keto-, 3 Beta-hydroxy-, 3alpha-hydroxy-, 3 Beta-hydroxy-12-keto-, 3alpha-hydroxy-12-keto-, 3 Beta,12alpha-dihydroxy-, and 3alpha,12alpha-dihydroxy-5 Beta-cholanoic acids was confirmed. Evidence was obtained for the presence of two bile acids having at least one hydroxyl group at a carbon atom other than C(3), C(7), or C(12).
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Citations
Cited by
Bile salt biotransformations by human intestinal bacteria
Ridlon, J. M., Kang, D.-J., & Hylemon, P. B. (2006) · Journal of Lipid Research · doi:10.1194/jlr.r500013-jlr200 · Cited by 2,829
Colonic Secretion of Water and Electrolytes Induced by Bile Acids: Perfusion Studies in Man
Mekhjian, H. S., Phillips, S. F., & Hofmann, A. F. (1971) · Journal of Clinical Investigation · doi:10.1172/jci106644 · Cited by 584
Blunt, J. W., Copp, B. R., Munro, M. H. G., Northcote, P. T., & Prinsep, M. R. (2011) · Nat. Prod. Rep. · doi:10.1039/c005001f · Cited by 470
General methods for the analysis of metabolic profiles of bile acids and related compounds in feces.
Setchell, K. D., Lawson, A. M., Tanida, N., & Sjövall, J. (1983) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37923-2 · Cited by 285
Self-citationHuman cecal bile acids: concentration and spectrum
Hamilton, J. P., Xie, G., Raufman, J.-P., Hogan, S., Griffin, T. L., Packard, C. A., Chatfield, D. A., Hagey, L. R., Steinbach, J. H., & Hofmann, A. F. (2007) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00027.2007 · Cited by 263
Metadata
- Authors
- Eneroth, P., Gordon, B., Ryhage, R., & Sjövall, J.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1966-07
- Volume
- 7
- Issue
- 4
- Pages
- 511-523
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2187322304
- Cited by
- 183
- References
- 0
- FWCI
- 32.15
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Eneroth, P., Gordon, B., Ryhage, R., & Sjövall, J. (1966). Identification of mono- and dihydroxy bile acids in human feces by gas-liquid chromatography and mass spectrometry. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)39261-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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