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Cited by 557

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

Open access
Gamble, W, Vaughan, M, Kruth, H S, Avigan, J·1978·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)40693-5
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Abstract

Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with p-hydroxyphenylacetic acid to yield a stable fluorescent product. Cholesterol ester hydrolase is included for determination of total cholesterol. When samples of sonified cell suspensions are used directly, the extraction of lipids is avoided, permitting one person to carry out analyses of 30 or more subcultures in one day.

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Metadata

Authors
Gamble, W., Vaughan, M., Kruth, H. S., & Avigan, J.
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1978-11
Volume
19
Issue
8
Pages
1068-1070
ISSN
0022-2275 (ISSN-L)
DOI
10.1016/s0022-2275(20)40693-5
OpenAlex
W2165482355
Cited by
557
References
1
FWCI
12.39
Type
journal article
Language
en
Open access
hybrid

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.