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Methods for the determination of adipose cell size in man and animals

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Hirsch, Jules, Gallian, E.·1968·Journal of Lipid Research

Abstract

Four methods for the sizing of adipose cells in small samples of human or animal adipose tissue are compared. These methods depend on the preparation of cell suspensions by incubation of the tissue with collagenase or by prolonged fixation with osmium tetroxide and separation of the fixed cells. A Coulter electronic counter was used to count and size the suspended cells and a Zeiss particle size analyzer for the sizing of cells in photomicrographs. The use of the Coulter counter to count cells in a suspension derived from a known amount of tissue and subjected to osmium tetroxide fixation is recommended for accuracy and general applicability to adipose cells of all sizes in man and animals.

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Metadata

Authors
Hirsch, J., & Gallian, E.
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1968-01
Volume
9
Issue
1
Pages
110-119
ISSN
0022-2275 (ISSN-L)
OpenAlex
W2189424535
Cited by
890
References
0
FWCI
28.24
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Methods for the determination of adipose cell size in man and animals. https://app.cochat.ai/papers/10.1016/s0022-2275(20)43151-7
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Hirsch, J., & Gallian, E. (1968). Methods for the determination of adipose cell size in man and animals. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)43151-7

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