The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
[16] Purification of proteins using polyhistidine affinity tags
Open accessOverview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Metal chelate affinity chromatography, a new approach to protein fractionation
PORATH, J., CARLSSON, J., OLSSON, I., & BELFRAGE, G. (1975) · Nature · doi:10.1038/258598a0 · Cited by 2,068
Current Protocols in Protein Science
(2018) · Current Protocols in Protein Science · doi:10.1002/(issn)1934-3663 · Cited by 1,189
Hochuli, E., Döbeli, H., & Schacher, A. (1987) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)93969-4 · Cited by 1,112
Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R., & Stüber, D. (1988) · Nature Biotechnology · doi:10.1038/nbt1188-1321 · Cited by 1,046
Immobilized metal ion affinity chromatography
Porath, J. (1992) · Protein Expression and Purification · doi:10.1016/1046-5928(92)90001-d · Cited by 644
Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro.
Robbins, D. J., Zhen, E., Owaki, H., Vanderbilt, C. A., Ebert, D., Geppert, T. D., & Cobb, M. H. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53507-9 · Cited by 497
Current protocols in protein science
Howland, J. L. (1996) · Biochemical Education · doi:10.1016/s0307-4412(96)80025-2 · Cited by 462
Janknecht, R., de Martynoff, G., Lou, J., Hipskind, R. A., Nordheim, A., & Stunnenberg, H. G. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.20.8972 · Cited by 451
Crystal structure of TFIID TATA-box binding protein
Nikolov, D. B., Hu, S.-H., Lin, J., Gasch, A., Hoffmann, A., Horikoshi, M., Chua, N.-H., Roeder, R. G., & Burley, S. K. (1992) · Nature · doi:10.1038/360040a0 · Cited by 405
Affinity Fusion Strategies for Detection, Purification, and Immobilization of Recombinant Proteins
Nilsson, J., Ståhl, S., Lundeberg, J., Uhlén, M., & Nygren, P.-Å. (1997) · Protein Expression and Purification · doi:10.1006/prep.1997.0767 · Cited by 332
Cited by
Recombinant protein expression in Escherichia coli: advances and challenges
Rosano, G. L., & Ceccarelli, E. A. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00172 · Cited by 2,556
Selective Covalent Protein Immobilization: Strategies and Applications
Wong, L. S., Khan, F., & Micklefield, J. (2009) · Chemical Reviews · doi:10.1021/cr8004668 · Cited by 489
Liu, X., Salokas, K., Tamene, F., Jiu, Y., Weldatsadik, R. G., Öhman, T., & Varjosalo, M. (2018) · Nature Communications · doi:10.1038/s41467-018-03523-2 · Cited by 258
Biochemical properties of glycerol kinase from the hypersaline-adapted archaeon Haloferax volcanii
Sanchez, K. M., & Maupin-Furlow, J. A. (2025) · Applied and Environmental Microbiology · doi:10.1128/aem.00886-25 · Cited by 5
Metadata
- Authors
- Bornhorst, J. A., & Falke, J. J.
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2000
- Volume
- 326
- Pages
- 245-254
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W2097771231
- Cited by
- 573
- References
- 18
- FWCI
- 3.84
- Type
- book chapter
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: [16] Purification of proteins using polyhistidine affinity tags. https://app.cochat.ai/papers/10.1016/s0076-6879(00)26058-8
- The paper
- Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of proteins using polyhistidine affinity tags. Methods in Enzymology. https://doi.org/10.1016/s0076-6879(00)26058-8
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.