The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.
Open accessAbstract
Vaccinia virus has been used as a vector to express foreign genes for the production of functional and posttranslationally modified proteins. A procedure is described here that allows the rapid native purification of vaccinia-expressed proteins fused to an amino-terminal tag of six histidines. Extracts from cells infected with recombinant vaccinia virus are loaded onto Ni2+.nitrilotriacetic acid (Ni2+.NTA)-agarose and histidine-tagged proteins are selectively eluted with imidazole-containing buffers. In the case of the human serum response factor (SRF), a transcription factor involved in the regulation of the c-fos protooncogene, the vaccinia-expressed histidine-tagged SRF (SRF-6His) could be purified solely by this step to greater than 95% purity. SRF-6His was shown to resemble authentic SRF by functional criteria: it was transported to the nucleus, bound specifically the c-fos serum response element, interacted with the p62TCF protein to form a ternary complex, and stimulated in vitro transcription from the serum response element. Thus, the combination of vaccinia virus expression and affinity purification by Ni2+.NTA chromatography promises to be useful for the production of proteins in a functional and posttranslationally modified form.
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Cites
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,366
Norman, C., Runswick, M., Pollock, R., & Treisman, R. (1988) · Cell · doi:10.1016/0092-8674(88)90244-9 · Cited by 957
Shaw, P. E., Schröter, H., & Nordheim, A. (1989) · Cell · doi:10.1016/0092-8674(89)90579-5 · Cited by 473
Self-citationFalkner, F. G., & Moss, B. (1988) · Journal of Virology · doi:10.1128/jvi.62.6.1849-1854.1988 · Cited by 316
Prywes, R., Dutta, A., Cromlish, J. A., & Roeder, R. G. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.19.7206 · Cited by 173
Amino‐terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication.
Gounari, F., Francesco, R. D., Schmitt, J., van der Vliet, P. C., Cortese, R., & Stunnenberg, H. G. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb08143.x · Cited by 152
Self-citationSchröter, H., Mueller, C. G., Meese, K., & Nordheim, A. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb08218.x · Cited by 137
Self-citationGrowth factor-induced gene expression: the ups and downs of c-fos regulation.
Rivera, V. M., & Greenberg, M. E. (1990) · PubMed · Cited by 95
Multiple Basal Promoter Elements Determine the Level of Human c- fos Transcription
Runkel, L., Shaw, P. E., Herrera, R. E., Hipskind, R. A., & Nordheim, A. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.3.1270-1280.1991 · Cited by 48
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The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway
Bucci, C., Parton, R. G., Mather, I. H., Stunnenberg, H., Simons, K., Hoflack, B., & Zerial, M. (1992) · Cell · doi:10.1016/0092-8674(92)90306-w · Cited by 1,373
Self-citation[16] Purification of proteins using polyhistidine affinity tags
Bornhorst, J. A., & Falke, J. J. (2000) · Methods in Enzymology · doi:10.1016/s0076-6879(00)26058-8 · Cited by 573
Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex.
Field, C. M., & Alberts, B. M. (1995) · The Journal of cell biology · doi:10.1083/jcb.131.1.165 · Cited by 438
Luo, J., Wang, Y., Yasuda, R. P., Dunah, A. W., & Wolfe, B. B. (1997) · Molecular Pharmacology · doi:10.1124/mol.51.1.79 · Cited by 401
Kaech, S. M., Whitfield, C. W., & Kim, S. K. (1998) · Cell · doi:10.1016/s0092-8674(00)81735-3 · Cited by 375
Metadata
- Authors
- Janknecht, R., de Martynoff, G., Lou, J., Hipskind, R. A., Nordheim, A., & Stunnenberg, H. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-10-15
- Volume
- 88
- Issue
- 20
- Pages
- 8972-8976
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2033303857
- Cited by
- 451
- References
- 9
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Janknecht, R., de Martynoff, G., Lou, J., Hipskind, R. A., Nordheim, A., & Stunnenberg, H. G. (1991). Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.20.8972
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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