The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Chapter 10 Monitoring Autophagy by Electron Microscopy in Mammalian Cells
Abstract
Electron microscopy remains one of the most accurate methods for the detection of autophagy and quantification of autophagic accumulation. Compared to fluorescence microscopy, the resolution of transmission electron microscopy is superior. In this chapter we describe the fine structure of early and late autophagic compartments in mammalian cells. Instructions are given for the preparation of samples for conventional electron microscopy using three different protocols suitable for cultured cells and animal tissues. We also introduce tomography as a tool to study the three-dimensional morphology of autophagic organelles and show the morphology of a phagophore as an example. Finally, we describe a protocol for the quantification of autophagic compartments by electron microscopy and point counting.
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“double- membraned autophagosomes were identified according to well established criteria”
From Nilotinib restores memory function by preventing dopaminergic neuron degeneration in a mouse model of Alzheimer’s Disease (2021) · cites this paper as Ylä-Anttila et al., 2009
Citations
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Research works printed but not linked: 1
- Eskelinen, 2008, Fine structure of the autophagosome, Methods Mol. Biol., 445, 11, 10.1007/978-1-59745-157-4_2 · DOI
Cited by
Sorted by citations
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
“1).(48) The sequential morphological changes during the autophagic process can be followed by TEM.”
Guidelines for the use and interpretation of assays for monitoring autophagy
Klionsky, D. J., Abdalla, F. C., Abeliovich, H., Abraham, R. T., Acevedo-Arozena, A., Adeli, K., Agholme, L., Agnello, M., Agostinis, P., Aguirre-Ghiso, J. A., et al. (2012) · Autophagy · doi:10.4161/auto.19496 · Cited by 3,996
“2A and B).(43,47) It should be emphasized that not all vesicles containing electrondense amorphous material are autolysosomes.”
BackgroundGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationAutophagy: assays and artifacts
Barth, S., Glick, D. R., & Macleod, K. F. (2010) · The Journal of Pathology · doi:10.1002/path.2694 · Cited by 819
“Some efforts have been made to quantify autophagy by TEM, for example, by measuring the ratio of early to late autophagic compartments or autophagic volume as a percentage of cytoplasmic volume [9], but again this depends on the trained eye and is arguably subjective.”
BackgroundDeacetylation of Nuclear LC3 Drives Autophagy Initiation under Starvation
Huang, R., Xu, Y., Wan, W., Shou, X., Qian, J., You, Z., Liu, B., Chang, C., Zhou, T., Lippincott-Schwartz, J., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2014.12.013 · Cited by 662
Metadata
- Authors
- Ylä‐Anttila, P., Vihinen, H., Jokitalo, E., & Eskelinen, E.
- Affiliations
- University of Helsinki (FI)
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2009
- Volume
- 452
- Pages
- 143-164
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W1594293927
- Cited by
- 257
- References
- 26
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 13 · 2023: 6 · 2022: 14 · 2021: 14 · 2020: 19 · 2019: 27 · 2018: 17 · 2017: 25 · 2016: 19 · 2015: 18 · 2014: 20 · 2013: 17 · 2012: 19
- FWCI
- 37.92
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- autophagy, phagophore, electron microscopy specimen preparation, electron tomography, three-dimensional morphology, point counts, mammalian cells
- MeSH terms
- Animals, Autophagy, Cells, Cultured, Microscopy, Electron, Transmission, Mice
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