[10] Quantitation of apolipoprotein E
Abstract
Quantitation of apoE has proved to be extremely useful in studies of the regulation of apoE synthesis and metabolism. Measurement of serum apoE and/or its distribution among the lipoprotein classes may have clinical utility, although this remains to be established. Some of the unique properties of apoE such as its genetic, chemical, and structural heterogeneity, its propensity to self-associate, and its ability to freely exchange on the surfaces of a wide variety of lipoprotein classes are factors that should be considered in measurements of apoE. The availability of commercial kits and reagents for human apoE quantitation make the development of apoE immunoassays readily achievable in most research and clinical laboratories.
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Detergent extraction of plasma, CSF, and brain samples before quantification precluded well-known issues of apolipoprotein detection in aqueous solutions.
“We also detergent extracted plasma, CSF and brain samples before quantification, thus precluding well known issues of apolipoprotein detection in aqueous solutions (Krul and Cole, 1996;Ramaswamy et al., 2005).”
From Impact of Apolipoprotein E (ApoE) Polymorphism on Brain ApoE Levels (2008) · cites this paper as Krul and Cole, 1996
A detergent-rich lysis buffer has been shown to inhibit apoE oligomerization and fully release lipid-bound apoE epitopes that, if not exposed, may hamper accurate detection of apoE levels by immunoassay.
“Such a detergent-rich lysis buffer has been shown to inhibit apoE oligomerization and fully release the lipid-bound apoE epitopes which, if not exposed, may hamper accurate detection of apoE levels by immunoassay (Krul and Cole, 1996;Ramaswamy et al., 2005).”
From Impact of Apolipoprotein E (ApoE) Polymorphism on Brain ApoE Levels (2008) · cites this paper as Krul and Cole, 1996
Cites
Apolipoprotein E: Structure-Function Relationships
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Three-Dimensional Structure of the LDL Receptor-Binding Domain of Human Apolipoprotein E
Wilson, C., Wardell, M. R., Weisgraber, K. H., Mahley, R. W., & Agard, D. A. (1991) · Science · doi:10.1126/science.2063194 · Cited by 678 · see citing works
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Protection against Alzheimer's disease with apoE ∈2
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Impact of Apolipoprotein E (ApoE) Polymorphism on Brain ApoE Levels
Riddell, D. R., Zhou, H., Atchison, K., Warwick, H. K., Atkinson, P. J., Jefferson, J., Xu, L., Aschmies, S., Kirksey, Y., Hu, Y., et al. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.1972-08.2008 · Cited by 342 · see citing works
APOE4-specific Changes in Aβ Accumulation in a New Transgenic Mouse Model of Alzheimer Disease
Youmans, K. L., Tai, L. M., Nwabuisi-Heath, E., Jungbauer, L., Kanekiyo, T., Gan, M., Kim, J., Eimer, W. A., Estus, S., Rebeck, G. W., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.407957 · Cited by 259 · see citing works
Huynh, T.-P. V., Wang, C., Tran, A. C., Tabor, G. T., Mahan, T. E., Francis, C. M., Finn, M. B., Spellman, R., Manis, M., Tanzi, R. E., et al. (2019) · Molecular Neurodegeneration · doi:10.1186/s13024-019-0337-1 · Cited by 139 · see citing works
- Authors
- Krul, E. S., & Cole, T. G.
- Affiliations
- Monsanto (United States) (US)
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 1996
- Volume
- 263
- Pages
- 170-187
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W1479719804
- Cited by
- 9
- References
- 4
- Citations by year
- 2019: 1 · 2012: 1
- FWCI
- 7.17
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- apolipoprotein E, immunoassay
- MeSH terms
- Animals, Apolipoproteins E, Biological Transport, Carrier Proteins, Cholesterol, Chylomicrons, Enzyme-Linked Immunosorbent Assay, Glycoproteins, Humans, Hyperlipoproteinemias, Lipase, Lipoproteins, Lipoproteins, VLDL, Polymorphism, Genetic, Receptors, LDL, Mice, Cholesterol Ester Transfer Proteins
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