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Structure of Functionally Activated Small Ribosomal Subunit at 3.3 Å Resolution
Open accessAbstract
The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.
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“These changes could be mediated by S12, which is in close proximity to the top of h44 (43, 58) and also appears to interact with Tet(O) (45).”
From Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance (2003) · cites this paper as [43]
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Metadata
- Authors
- Schluenzen, F., Tocilj, A., Zarivach, R., Harms, J., Gluehmann, M., Janell, D., Bashan, A., Bartels, H., Agmon, I., Franceschi, F., & Yonath, A.
- Affiliations
- Weizmann Institute of Science (IL)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2000-09
- Volume
- 102
- Issue
- 5
- Pages
- 615-623
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2092617311
- Cited by
- 979
- References
- 72
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 18 · 2023: 28 · 2022: 18 · 2021: 26 · 2020: 27 · 2019: 18 · 2018: 22 · 2017: 25 · 2016: 18 · 2015: 31 · 2014: 26 · 2013: 27 · 2012: 43
- FWCI
- 28.84
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM-34360, R01GM034360)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Keywords
- small ribosomal subunit, ribosome structure, Thermus thermophilus, decoding center, tRNA binding, mRNA translocation, translation fidelity, ribosomal proteins
- MeSH terms
- Binding Sites, Models, Molecular, Nucleic Acid Conformation, Protein Conformation, Ribosomes, RNA, Bacterial, RNA, Messenger, RNA, Ribosomal, 16S, RNA, Transfer, Structure-Activity Relationship, Thermus thermophilus, Crystallography, X-Ray, Base Pairing
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