Cited by 204Developmental Biology and Gene RegulationWnt/β-catenin signaling in development and cancerGene Regulatory Network Analysis

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The Interpretation of Position in a Morphogen Gradient as Revealed by Occupancy of Activin Receptors

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Dyson, Steven, Gurdon, J.B·1998·Cell

DOI 10.1016/s0092-8674(00)81185-x

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Abstract

Xenopus blastula cells activate different mesodermal genes as a concentration-dependent response to activin, which behaves like a morphogen. To understand how cells recognize morphogen concentration, we have bound naturally labeled activin to cells and related this to choice of gene activation. We find that the increasing occupancy of a single receptor type can cause cells to switch gene expression. Cells sense ligand concentration by the absolute number of occupied receptors per cell (100 and 300 molecules of bound activin induce Xbra and Xgsc, respectively, i.e., 2% and 6% of the total receptors) and not by a ratio of occupied to unoccupied receptors. The long duration of occupancy explains a previously described ratchet effect. Our results suggest a new concept of morphogen gradient formation and interpretation that is particularly well suited to the needs of early development.

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Authors: Dyson, S., & Gurdon, J. B.
Affiliations: University of Cambridge (GB)
Corresponding author: Gurdon, J.B
ORCID: Gurdon, J.B
Venue: Cell
Publisher: Elsevier BV
Published: 1998-05
Volume: 93
Issue: 4
Pages: 557-568
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/s0092-8674(00)81185-x
OpenAlex: W2110126772
Cited by: 204
References: 53
Citations by year: 2026: 1 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 2 · 2017: 2 · 2016: 7 · 2015: 4 · 2014: 5 · 2013: 7 · 2012: 3
FWCI: 6.60
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 5556 d)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Funders: Wellcome Trust
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords: activin, morphogen gradients, receptor occupancy, Xbra, ratchet effect, gene expression switching, early embryonic development
MeSH terms: Brachyury Protein, Animals, Blastocyst, DNA-Binding Proteins, Fetal Proteins, Inhibins, Ligands, Oligopeptides, Oocytes, Peptides, Repressor Proteins, RNA, Messenger, Transcription Factors, Xenopus, Signal Transduction, Transcriptional Activation, Receptors, Growth Factor, Homeodomain Proteins, Gene Expression Regulation, Developmental, T-Box Domain Proteins, Activins, Activin Receptors, Goosecoid Protein

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The Interpretation of Position in a Morphogen Gradient as Revealed by Occupancy of Activin Receptors

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