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Improved method for i.r. determination of the degree of N-acetylation of chitosan
Open accessAbstract
Three published i.r. absorption band ratios for determining the % N-acetylation of chitosan have been used to follow the rate of alkaline deacetylation of chitin. Only one, the A1655/A3450 ratio, gives the expected rate of deacetylation curve. Consideration of the various i.r. ratios has led to a new relationship, % N-acetylation = (A1655/A3450) × 115, where the absorbance of the amide I band at approximately 1655 cm−1 is determined using the baseline proposed previously. The values obtained using this proposed relationship agree closely with those obtained from dye adsorption measurements for chitosans having % N-acetylation values in the range 0–55%.
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Cited by
A review of chitin and chitosan applications
Ravi Kumar, M. N. V. (2000) · Reactive and Functional Polymers · doi:10.1016/s1381-5148(00)00038-9 · Cited by 6,009
Shigemasa, Y., Matsuura, H., Sashiwa, H., & Saimoto, H. (1996) · International Journal of Biological Macromolecules · doi:10.1016/0141-8130(95)01079-3 · Cited by 438
Chitin-Silk Fibroin Interactions: Relevance to Calcium Carbonate Formation in Invertebrates
Falini, G., Weiner, S., & Addadi, L. (2003) · Calcified Tissue International · doi:10.1007/s00223-002-1055-0 · Cited by 101
Preparation of chitosan from waste shrimp shells fermented with Paenibacillus jamilae BAT1
Taser, B., Ozkan, H., Adiguzel, A., Orak, T., Baltaci, M. O., & Taskin, M. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.05.062 · Cited by 30
Metadata
- Authors
- Baxter, A., Dillon, M., Anthony Taylor, K. D., & Roberts, G. A. F.
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 1992-06
- Volume
- 14
- Issue
- 3
- Pages
- 166-169
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2079316499
- Cited by
- 522
- References
- 0
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- green
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- Baxter, A., Dillon, M., Anthony Taylor, K. D., & Roberts, G. A. F. (1992). Improved method for i.r. determination of the degree of N-acetylation of chitosan. International Journal of Biological Macromolecules. https://doi.org/10.1016/s0141-8130(05)80007-8
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