Preparation of chitosan from waste shrimp shells fermented with Paenibacillus jamilae BAT1
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In this study, chitin extraction from shrimp shell powder (SSP) using locally isolated Paenibacillus jamilae BAT1 (GenBank: MN176658), the preparation of chitosan from the extracted chitin, and the characterization and biological activity (antimicrobial and antioxidant) of the prepared chitosan (PC) were investigated. It was determined that P. jamilae BAT1 did not have chitinase activity but showed high protease activity and protein removal potential. Optimum pH, shell concentration and incubation time for deproteinization were determined as 7.0, 60 g/L and 4 days, respectively. Addition of KH 2 PO 4 or MgSO 4 did not affect chitin extraction and deproteinization yield. The maximum yields of deproteinization, demineralization and chitin extraction yields were 87.67, 41.95 and 24.5%, respectively. The viscosity-average molecular weight of PC was determined as 1.41 × 10 5 g/mol. The deacetylation degree of PC (86%) was found to be higher that of commercial chitosan (CC) (78%). DPPH scavenging activity of PC (IC 50 0.59 mg/mL) was higher than that of CC (IC 50 3.72 mg/mL). PC was found to have higher antimicrobial activity against the bacteria E. coli and S. aureus and the yeast C. albicans when compared to CC. This is the first study on the use of the bacterium P. jamilae in biological chitin extraction.
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Fermented waste shrimp shells with Paenibacillus jamilae BAT1 have antimicrobial and antioxidant properties.
“Waste shrimp shells Paenibacillus jamilae BAT1 Antimicrobial and antioxidant [167]”
From Use of Waste from the Food Industry and Applications of the Fermentation Process to Create Sustainable Cosmetic Products: A Review (2024) · cites this paper as [167]
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- Authors
- Taser, B., Ozkan, H., Adiguzel, A., Orak, T., Baltaci, M. O., & Taskin, M.
- Affiliations
- Atatürk University (TR)
- ORCID
- Orak, Tugba
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2021-07
- Volume
- 183
- Pages
- 1191-1199
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W3160037123
- Cited by
- 30
- References
- 95
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 6 · 2023: 10 · 2022: 5 · 2021: 1
- FWCI
- 1.44
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Ağrı İbrahim Çeçen University Institutional Repository (submittedVersion, cc-by-nc-nd)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Studies on Chitinases and Chitosanases
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- chitosan, shrimp shell waste, chitin extraction, deproteinization, demineralization, degree of deacetylation, proteolytic activity, antimicrobial activity, antioxidant activity, DPPH radical scavenging, Escherichia coli, Staphylococcus aureus, Candida albicans, molecular weight
- MeSH terms
- Animals, Anti-Infective Agents, Bacterial Proteins, Candida albicans, Chitinases, Escherichia coli, Fermentation, Microbial Sensitivity Tests, Molecular Weight, Peptide Hydrolases, Staphylococcus aureus, Penaeidae, Chitosan, Paenibacillus, Animal Shells
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