Cited by 30Nanocomposite Films for Food PackagingStudies on Chitinases and ChitosanasesCollagen: Extraction and Characterization

Preparation of chitosan from waste shrimp shells fermented with Paenibacillus jamilae BAT1

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Taser, Behiye, Ozkan, Hakan, Adiguzel, Ahmet, Orak, Tugba, Baltaci, Mustafa Ozkan, Taskin, Mesut·2021·International Journal of Biological Macromolecules

Abstract

In this study, chitin extraction from shrimp shell powder (SSP) using locally isolated Paenibacillus jamilae BAT1 (GenBank: MN176658), the preparation of chitosan from the extracted chitin, and the characterization and biological activity (antimicrobial and antioxidant) of the prepared chitosan (PC) were investigated. It was determined that P. jamilae BAT1 did not have chitinase activity but showed high protease activity and protein removal potential. Optimum pH, shell concentration and incubation time for deproteinization were determined as 7.0, 60 g/L and 4 days, respectively. Addition of KH 2 PO 4 or MgSO 4 did not affect chitin extraction and deproteinization yield. The maximum yields of deproteinization, demineralization and chitin extraction yields were 87.67, 41.95 and 24.5%, respectively. The viscosity-average molecular weight of PC was determined as 1.41 × 10 5 g/mol. The deacetylation degree of PC (86%) was found to be higher that of commercial chitosan (CC) (78%). DPPH scavenging activity of PC (IC 50 0.59 mg/mL) was higher than that of CC (IC 50 3.72 mg/mL). PC was found to have higher antimicrobial activity against the bacteria E. coli and S. aureus and the yeast C. albicans when compared to CC. This is the first study on the use of the bacterium P. jamilae in biological chitin extraction.

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Crossref · 10.1016/j.ijbiomac.2021.05.062

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Authors
Taser, B., Ozkan, H., Adiguzel, A., Orak, T., Baltaci, M. O., & Taskin, M.
Affiliations
Atatürk University (TR)
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2021-07
Volume
183
Pages
1191-1199
ISSN
0141-8130 (ISSN-L)
OpenAlex
W3160037123
Cited by
30
References
95
Citations by year
2026: 4 · 2025: 4 · 2024: 6 · 2023: 10 · 2022: 5 · 2021: 1
FWCI
1.44
Type
journal article
Language
en
Open access
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
  • Ağrı İbrahim Çeçen University Institutional Repository (submittedVersion, cc-by-nc-nd)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Studies on Chitinases and Chitosanases
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
Keywords
chitosan, shrimp shell waste, chitin extraction, deproteinization, demineralization, degree of deacetylation, proteolytic activity, antimicrobial activity, antioxidant activity, DPPH radical scavenging, Escherichia coli, Staphylococcus aureus, Candida albicans, molecular weight
MeSH terms
Animals, Anti-Infective Agents, Bacterial Proteins, Candida albicans, Chitinases, Escherichia coli, Fermentation, Microbial Sensitivity Tests, Molecular Weight, Peptide Hydrolases, Staphylococcus aureus, Penaeidae, Chitosan, Paenibacillus, Animal Shells

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