The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Role of lectins in the innate immunity of horseshoe crab
Abstract
We have purified five types of lectins, named tachylectins, from circulating hemocytes and hemolymph plasma of the Japanese horseshoe crab, Tachypleus tridentatus. Tachylectin-1 interacts with Gram-negative bacteria probably through 2-keto-3-deoxyoctonate, one of the constituents of lipopolysaccharides (LPS). Tachylectin-1 also binds to polysaccharides such as agarose and dextran with broad specificity. Tachylectin-2 binds to D-GlcNAc or D-GalNAc and recognizes staphylococcal lipoteichoic acids and LPS from several Gram-negative bacteria. In contrast, tachylectins-3 and -4 specifically bind to S-type LPS from several Gram-negative bacteria through a certain sugar moiety on the O-specific polysaccharides (O-antigens). Tachylectin-5 identified in hemolymph plasma has the strongest bacterial agglutinating activity in the five types of tachylectins, and exhibits broad specificity against acetyl group-containing substances. Thus, the innate immune system of horseshoe crab may recognize invading pathogens through a combinatorial method using lectins with different specificities against carbohydrates exposed on pathogens. An encounter of these lectins derived from hemocytes and hemolymph plasma at injured sites, in response to the stimulation of LPS, suggests that they serve synergistically to accomplish an effective host defense against invading microbes and foreign substances.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Citations
Cites
CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein
Wright, S. D., Ramos, R., Tobias, P. S., Ulevitch, R. J., & Mathison, J. C. (1990) · Science · doi:10.1126/science.1698311 · Cited by 3,996
Mannose-binding lectin: the pluripotent molecule of the innate immune system
TURNER, M. W. (1996) · Immunology Today · doi:10.1016/s0167-5699(96)80908-x · Cited by 709
Structure of pentameric human serum amyloid P component
Emsley, J., White, H. E., O'Hara, B. P., Oliva, G., Srinivasan, N., Tickle, I. J., Blundell, T. L., Pepys, M. B., & Wood, S. (1994) · Nature · doi:10.1038/367338a0 · Cited by 466
Three dimensional structure of human C-reactive protein
Shrive, A., Gheetham, G. M. T., Holden, D., Myles, D., Turnell, W., Volanakis, J., Pepys, M., Bloomer, A., & Greenhough, T. (1996) · Nature Structural Biology · doi:10.1038/nsb0496-346 · Cited by 349
New Directions in Invertebrate Immunology
Söderhäll, K., Iwanaga, S., & Vasta, G. R. (1996) · Cited by 342
Self-citationIwanaga, S., Kawabata, S., & Muta, T. (1998) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a021894 · Cited by 287
Self-citationThe role of hemolymph coagulation in innate immunity
Muta, T., & Iwanaga, S. (1996) · Current Opinion in Immunology · doi:10.1016/s0952-7915(96)80103-8 · Cited by 283
Self-citationBacterial Endotoxic Lipopolysaccharides
Morrison, D. C., & Ryan, J. L. (2024) · CRC Press eBooks · doi:10.1201/9781003574859 · Cited by 273
Limulin: a C-reactive protein from Limulus polyphemus.
Robey, F. A., & Liu, T. Y. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70074-x · Cited by 205
Immunology of Insects and Other Arthropods
Gupta, A. P. (2019) · CRC Press eBooks · doi:10.1201/9781351073431 · Cited by 190
Research works printed but not linked: 2
- Knirel, 1994, The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: a review, Biochemistry (Moscow), 59, 1325
- Holst, 1992, Chemical structure of the core region of lipopolysaccharide, vol. 1, 135
Cited by
Sorted by citations
Varki, A. (2017) · Glycobiology · doi:10.1093/glycob/cww086 · Cited by 2,635
“For example, the hemolymph of horseshoe crabs recognizes invaders through a combinatorial approach, using lectins with different specificities against glycans exposed on pathogens (782), allowing these organisms to survive almost unchanged for >100 million years, without the benefit of adaptive immunity.”
BackgroundRecent Advances in the Innate Immunity of Invertebrate Animals
Iwanaga, S., & Lee, B.-L. (2005) · BMB Reports · doi:10.5483/bmbrep.2005.38.2.128 · Cited by 757
Self-citationThe evolution and genetics of innate immunity
Kimbrell, D., & Beutler, B. (2001) · Nature reviews genetics · doi:10.1038/35066006 · Cited by 679
Animal lectins: a historical introduction and overview
Kilpatrick, D. C. (2002) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(02)00308-2 · Cited by 502
Early events in crustacean innate immunity
Lee, S. Y., & Söderhäll, K. (2002) · Fish & Shellfish Immunology · doi:10.1006/fsim.2002.0420 · Cited by 460
Metadata
- Authors
- Kawabata, S.-I., & Iwanaga, S.
- Affiliations
- Kyushu University (JP)
- Corresponding author
- Kawabata, Shun-ichiro
- Venue
- Developmental & Comparative Immunology
- Publisher
- Elsevier BV
- Published
- 1999-06
- Volume
- 23
- Issue
- 4-5
- Pages
- 391-400
- ISSN
- 0145-305X (ISSN-L)
- OpenAlex
- W2025265837
- Cited by
- 114
- References
- 27
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 4 · 2019: 1 · 2018: 6 · 2017: 1 · 2016: 7 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 4
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental & Comparative Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Core Research for Evolutional Science and Technology
- Naito Foundation
- Japan Science and Technology Corporation
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- lectins, horseshoe crab, Tachypleus tridentatus, innate immunity, hemocytes, lipopolysaccharide recognition, Gram-negative bacteria, bacterial agglutination, O-antigen, lipoteichoic acid, carbohydrate recognition, host defense
- MeSH terms
- Animals, Gram-Negative Bacteria, Hemocytes, Horseshoe Crabs, Immunity, Innate, Lipopolysaccharides, Teichoic Acids, Sequence Alignment, O Antigens, Lectins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
