Isolation and characterization of a novel human gene (NESH) which encodes a putative signaling molecule similar to e3B1 protein
Abstract
Using a conventional cloning technique, a novel full-length cDNA was isolated and sequenced from a human placental cDNA library. This cDNA consists of 2129 bp and has a predicted open reading frame encoding 366 amino acids. It possesses a Src homology 3 (SH3) motif, proline-rich region, serine-rich region and no catalytic domain, suggesting that it seems to be a signaling protein most similar to e3B1, an eps8 SH3 binding protein. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 17q21.3 near the marker D17S1795.
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NESH was initially isolated from a human placental cDNA library as a novel gene featuring an SH3 domain, along with proline-rich and serine-rich regions.
“NESH (NEw molecule containing SH3) was initially isolated from a human placental cDNA library as a novel gene featuring an SH3 domain, along with proline-rich and serine-rich regions.”
From The WAVE complex in developmental and adulthood brain disorders (2025) · cites this paper as [142]
Due to its sequence similarity to ABI1 and ABI2, NESH was subsequently incorporated into the ABI family and designated ABI3.
“Due to its sequence similarity to ABI1 and ABI2, it was subsequently incorporated into the ABI family and designated ABI3142.”
From The WAVE complex in developmental and adulthood brain disorders (2025) · cites this paper as [142]
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Grove, M., Demyanenko, G., Echarri, A., Zipfel, P. A., Quiroz, M. E., Rodriguiz, R. M., Playford, M., Martensen, S. A., Robinson, M. R., Wetsel, W. C., et al. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.24.10905-10922.2004 · Cited by 136 · see citing works
The NESH/Abi-3-based WAVE2 complex is functionally distinct from the Abi-1-based WAVE2 complex
Sekino, S., Kashiwagi, Y., Kanazawa, H., Takada, K., Baba, T., Sato, S., Inoue, H., Kojima, M., & Tani, K. (2015) · Cell Communication and Signaling · doi:10.1186/s12964-015-0119-5 · Cited by 43 · see citing works
Microglia express ABI3 in the brains of Alzheimer's disease and Nasu-Hakola disease
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Deletion of Abi3/Gngt2 influences age-progressive amyloid β and tau pathologies in distinctive ways
Ibanez, K. R., McFarland, K. N., Phillips, J., Allen, M., Lessard, C. B., Zobel, L., De La Cruz, E. G., Shah, S., Vo, Q., Wang, X., et al. (2022) · Alzheimer's Research & Therapy · doi:10.1186/s13195-022-01044-1 · Cited by 18 · see citing works
The WAVE complex in developmental and adulthood brain disorders
Kim, H.-G., Berdasco, C., Nairn, A. C., & Kim, Y. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01386-w · Cited by 8 · see citing works
- Authors
- Miyazaki, K., Matsuda, S., Ichigotani, Y., Takenouchi, Y., Hayashi, K., Fukuda, Y., Nimura, Y., & Hamaguchi, M.
- Affiliations
- Nagoya University (JP)
- Corresponding author
- Matsuda, Satoru
- Venue
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
- Publisher
- Elsevier BV
- Published
- 2000-09
- Volume
- 1493
- Issue
- 1-2
- Pages
- 237-241
- ISSN
- 0167-4781 (ISSN-L)
- OpenAlex
- W2044148332
- Cited by
- 32
- References
- 0
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 4 · 2020: 1 · 2017: 1 · 2015: 1 · 2014: 1 · 2012: 4
- FWCI
- 0.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- nesh, novel human gene, proline-rich region, serine-rich region, signaling proteins, placenta, cDNA cloning, gene mapping, radiation hybrid mapping
- MeSH terms
- Amino Acid Sequence, Base Sequence, Carrier Proteins, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 17, Cloning, Molecular, Cytoskeletal Proteins, Genes, Humans, Molecular Sequence Data, Gene Library, Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, DNA, Complementary, src Homology Domains, Protein Sorting Signals, Adaptor Proteins, Signal Transducing
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