Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 426

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs

Open access
Lewis, Tom, Nichols, Peter D, McMeekin, Tom A·2000·Journal of Microbiological Methods
DOI 10.1016/s0167-7012(00)00217-7
Open this paper in CoChat — free account

Abstract

The effect of different extraction techniques on the recovery of fatty acids from freeze-dried biomass of two lipid-producing microheterotrophs was examined. Two procedures were used: the extraction of lipids from biomass followed by transesterification of the fatty acids (extraction-transesterification); and the direct transesterification of biomass to produce fatty acid methyl esters (i.e. without the initial extraction step). Variable factors in the extraction-transesterification experiment were the sequence in which solvents were added to the samples, the relative amount of methanol in the solvent mix, and sonication of biomass while in the solvent mix. Variable factors in the direct transesterification experiment were sample size, and reaction duration. Statistical analysis of data (level of significance P<0.05) showed that: (1) extraction of total fatty acids prior to transesterification was significantly more efficient when solvents were added in the order of increasing polarity; (2) neither sonication nor increasing the proportion of methanol in the extraction solvent significantly affected extraction of fatty acids prior to transesterification; (3) efficiency of direct transesterification of fatty acids increased significantly with reaction time; (4) efficiency of direct transesterification of fatty acids was not significantly affected by sample size; (5) the most efficient method for extraction of fatty acids prior to transesterification yielded significantly less fatty acids than the most effective direct transesterification method. While the study examined only two strains, our results suggest that fatty acid analysis methodology for microheterotrophs under consideration for biotechnological exploitation requires optimisation and validation.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/s0167-7012(00)00217-7

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 1 references

Research works printed but not linked: 25

  1. Allen, 1999, Monounsaturated but not polyunsaturated fatty acids are required for growth of the deep-sea bacterium Photobacterium profundum SS9 at high pressure and low temperature, Appl. Environ. Microbiol., 65, 1710, 10.1128/AEM.65.4.1710-1720.1999 · DOI · Paper page
  2. Bajpai, 1991, Optimization of production of docosahexaenoic acid (DHA) by Thraustochytrium aureum ATCC 34304, J. Am. Oil Chem. Soc., 68, 509, 10.1007/BF02663823 · DOI · Paper page
  3. Bohnert, 1997, Direct esterification method for analysis of long-chain polyunsaturated fatty acids (LC–PUFAs) in infant formulae, Z. Lebensm. Unters. Forsch. A, 204, 27, 10.1007/s002170050031 · doi:10.1007/s002170050031
  4. Dionisi, 1999, Stability of cyclopropane and conjugated linoleic acids during fatty acid quantification in lactic acid bacteria, Lipids, 34, 1107, 10.1007/s11745-999-0462-8 · DOI
  5. Dunstan, 1993, The effect of lyophilization on the solvent extraction of lipid classes, fatty acids and sterols from the oyster Crassostrea gigas, Lipids, 28, 937, 10.1007/BF02537504 · DOI
  6. Dunstan, 1992, Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 2. Lipid classes and fatty acids, J. Exp. Mar. Biol. Ecol., 161, 115, 10.1016/0022-0981(92)90193-E · DOI · Paper page
  7. Eroshin, 1996, Studies on arachidonic acid production by Mortierella fungi — a microbiological method for selecting arachidonic acid producers, Microbiology, 65, 26
  8. Gandhi, 1991, Production of the polyunsaturated fatty acids arachidonic acid and eicosapentaenoic acid by the fungus Pythium ultimum, J. Gen. Microbiol., 137, 1825, 10.1099/00221287-137-8-1825 · doi:10.1099/00221287-137-8-1825
  9. Garces, 1993, One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissue, Analyt. Biochem., 211, 139, 10.1006/abio.1993.1244 · DOI · Paper page
  10. Guckert, 1988, Lipid solvent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella, J. Microbiol. Methods, 8, 139, 10.1016/0167-7012(88)90015-2 · DOI
  11. Guckert, 1988, Evaluation of a hexane/isopropanol lipid solvent system for analysis of bacterial phospholipids and application to chloroform-soluble Nuclepore (polycarbonate) membranes with retained bacteria, J. Microbiol. Methods, 8, 131, 10.1016/0167-7012(88)90014-0 · DOI
  12. Iida, 1996, Improvement of docosahexaenoic acid production in a culture of Thraustochytrium aureum by medium optimization, J. Ferment. Bioeng., 81, 76, 10.1016/0922-338X(96)83125-4 · DOI
  13. Leman, 1997, Oleaginous microorganisms: an assessment of the potential, 195, 10.1016/S0065-2164(08)70226-0 · DOI
  14. Lepage, 1984, Improved recovery of fatty acid through direct transesterification without prior extraction or purification, J. Lipid Res., 25, 1391, 10.1016/S0022-2275(20)34457-6 · DOI · Paper page
  15. Macnaughton, 1997, Rapid extraction of lipid biomarkers from pure culture and environmental samples using pressurized accelerated hot solvent extraction, J. Microbiol. Methods, 31, 19, 10.1016/S0167-7012(97)00081-X · doi:10.1016/s0167-7012(97)00081-x
  16. Mayberry, 1993, Sequential alkaline saponification acid hydrolysis esterification — a one-tube method with enhanced recovery of both cyclopropane and hydroxylated fatty acids, J. Microbiol. Methods, 18, 21, 10.1016/0167-7012(93)90068-S · doi:10.1016/0167-7012(93)90068-s
  17. Nakahara, 1996, Production of docosahexaenoic and docosapentaenoic acids by Schizochytrium sp. isolated from Yap Islands, J. Am. Oil Chem. Soc., 73, 1421, 10.1007/BF02523506 · DOI · Paper page
  18. Nichols, 1996, Enrichment of the rotifer Brachionus plicatilis fed an Antarctic bacterium containing polyunsaturated fatty acids, Aquaculture, 147, 115, 10.1016/S0044-8486(96)01391-9 · DOI
  19. Ratledge, 1993, Single cell oils — have they a biotechnological future?, Trends Biotechnol., 11, 278, 10.1016/0167-7799(93)90015-2 · DOI · Paper page
  20. Rodriguez-Ruiz, 1998, Rapid simultaneous lipid extraction and transesterification for fatty acid analyses, Biotechnol. Tech., 12, 689, 10.1023/A:1008812904017 · DOI · Paper page
  21. Sajbidor, 1990, Arachidonic acid production by Mortierella sp. S-17. Influence of C/N ratio, Biotechnol. Lett., 12, 455, 10.1007/BF01024404 · DOI
  22. Smedes, 1999, Revisiting the development of the Bligh and Dyer total lipid determination method, Mar. Pollution Bull., 38, 193, 10.1016/S0025-326X(98)00170-2 · DOI
  23. Sonnichsen, 1999, A rapid and quantitative method for total fatty acid analysis of fungi and other biological samples, Lipids, 34, 1347, 10.1007/s11745-999-0488-z · DOI
  24. White, 1979, Determination of the sedimentary microbial biomass by extractable lipid phosphate, Oecologia, 40, 51, 10.1007/BF00388810 · DOI · Paper page
  25. Yokochi, 1998, Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21, Appl. Microbiol. Biotechnol., 49, 72, 10.1007/s002530051139 · DOI · Paper page

Cited by

See all 426

Metadata

Authors
Lewis, T., Nichols, P. D., & McMeekin, T. A.
Venue
Journal of Microbiological Methods
Publisher
Elsevier BV
Published
2000-12
Volume
43
Issue
2
Pages
107-116
ISSN
0167-7012 (ISSN-L)
DOI
10.1016/s0167-7012(00)00217-7
OpenAlex
W2063878611
Cited by
426
References
1
FWCI
3.08
Type
journal article
Language
en
Open access
green
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs. https://app.cochat.ai/papers/10.1016/s0167-7012(00)00217-7
The paper
Lewis, T., Nichols, P. D., & McMeekin, T. A. (2000). Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs. Journal of Microbiological Methods. https://doi.org/10.1016/s0167-7012(00)00217-7

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.