The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Improved recovery of fatty acid through direct transesterification without prior extraction or purification.
Open accessAbstract
Methods currently in use for the quantitative measurement of fatty acids by gas-liquid chromatography after transesterification are usually lengthy and cumbersome. The technique described is a one-step reaction that is carried out in the same tube and bypasses all the extraction and purification steps. Recoveries of fatty acid and triglyceride standards (C6:0 to C24:1) were better than 96%. When the direct transesterification method was compared to the Folch extraction procedure, increases of fatty acid concentration of 11.4% and 15.8% were observed in human milk and adipose tissue, respectively. The method appears to be particularly advantageous for the recovery of the highly volatile medium chain triglycerides and there is no need to add an antioxidant to protect unsaturated lipids.
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Cites
Medium-chain triglycerides: an update
Bach, A., & Babayan, V. (1982) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/36.5.950 · Cited by 1,151
Cited by
Direct transesterification of all classes of lipids in a one-step reaction.
Lepage, G., & Roy, C. C. (1986) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38861-1 · Cited by 2,384
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Halim, R., Danquah, M. K., & Webley, P. A. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.01.001 · Cited by 1,043
Relationship of plasma polyunsaturated fatty acids to circulating inflammatory markers.
Ferrucci, L., Cherubini, A., Bandinelli, S., Bartali, B., Corsi, A., Lauretani, F., Martin, A., Andres-Lacueva, C., Senin, U., & Guralnik, J. M. (2006) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2005-1303 · Cited by 696
National Algal Biofuels Technology Roadmap
USDOE Office of Energy Efficiency and Renewable Energy (EERE), O. O. S. T. B. T. O. (., Ferrell, J., US Department of Energy (USDOE), W., & Sarisky-Reed, V. (2010) · doi:10.2172/1218560 · Cited by 470
Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2014.10.002 · Cited by 321
Metadata
- Authors
- Lepage, G., & Roy, C. C.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1984-12
- Volume
- 25
- Issue
- 12
- Pages
- 1391-1396
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W1933801203
- Cited by
- 1,021
- References
- 1
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lepage, G., & Roy, C. C. (1984). Improved recovery of fatty acid through direct transesterification without prior extraction or purification. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)34457-6
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