Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi
Abstract
Artemisia annua, well recognized for its production of antimalarial drug artemisinin, is seldom attacked by any of phytopathogenic fungi, which could be partially associated with the presence of endophytes. Present investigation is aiming at disclosing whether the endophytes inside A. annua produce antifungal substances. A total of 39 endophytes were isolated and fermented, and the ferment broth was evaluated in vitro for the antifungal activity against crop-threatening fungi Gaeumannomyces graminis var. tritici, Rhizoctonia cerealis, Helminthosporium sativum, Fusarium graminearum, Gerlachia nivalis and Phytophthora capsici. These plant pathogens are still causing wheat take-all, sharp eyespot, common rot, scab, snow mould, and pepper phytophthora blight, respectively. Out of 39 endophytes investigated, 21 can produce in vitro substances that are inhibitory to all or a few of the tested phytopathogens whereas the rest yielded nothing active. Moreover, the most active broth of endophyte IV403 was extracted with EtOAc and n-butanol, and comparisons of the antifungal activity of the extracts indicated that the major active metabolites were EtOAc-extractable.
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Lateral transfer of genes involved in taxol biosynthesis from Taxus brevifolium to an endophytic symbiont has been observed.
“Interest in endophytic fungi as a novel source of plant-derived drugs was first sparked by the observation of the lateral transfer of genes which are involved in the biosynthesis of taxol (paclitaxel) from Taxus brevifolium L. [356] - the plant which produces this important anti-cancer compound - to an endophytic symbiont.”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [356]
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The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao)
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- Authors
- Liu, C. H., Zou, W. X., Lu, H., & Tan, R. X.
- Affiliations
- Nanjing University (CN)
- Corresponding author
- Tan, Ren Xiang
- ORCID
- Tan, Ren Xiang
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2001-07
- Volume
- 88
- Issue
- 3
- Pages
- 277-282
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W2067177675
- Cited by
- 220
- References
- 12
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 2 · 2023: 2 · 2022: 9 · 2021: 16 · 2020: 9 · 2019: 14 · 2018: 9 · 2017: 6 · 2016: 9 · 2015: 11 · 2014: 10 · 2013: 14 · 2012: 15
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
- Keywords
- Artemisia annua, endophytes, antifungal activity, plant pathogenic fungi, Gaeumannomyces graminis var. tritici, Rhizoctonia cerealis, Bipolaris sorokiniana, Fusarium graminearum, Phytophthora capsici, wheat take-all, sharp eyespot, scab, snow mold, ethyl acetate extract, n-butanol extract, plant disease control
- MeSH terms
- Actinomycetales, Antifungal Agents, Fermentation, Fungi, Microbial Sensitivity Tests, Symbiosis, Artemisia
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