The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation
Open accessAbstract
PURPOSE: This study compared two methods of preparing platelet-rich plasma (PRP) gel and the levels of PDGF and TGFbeta in each preparation. MATERIALS AND METHODS: Platelet-rich plasma gel was prepared by centrifugation and clotted using the ITA gelling agent (Natrex Technologies Inc, Greenville, NC) or by the addition of thrombin and calcium chloride. The levels of platelet-derived growth factor (PDGF) and transforming growth factor beta (TGFbeta) generated by clot formation were assayed by enzyme-linked immunoassay (ELISA). RESULTS: Both methods of preparation yielded PRP gel in less than 30 minutes. However, the ITA preparation did not require thrombin to achieve adequate gel formation. The levels of PDGF and TGFbeta were similar regardless of which method was used for initiation of clot formation. CONCLUSION: Use of ITA for gel preparation is equivalent to using calcium chloride and thrombin, without the need for special equipment and the risk of coagulopathy.
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Cites
Growth factors for bone growth and repair: IGF, TGFβ and BMP
Linkhart, T. A., Mohan, S., & Baylink, D. J. (1996) · Bone · doi:10.1016/s8756-3282(96)00138-x · Cited by 440
Cited by
Okuda, K., Kawase, T., Momose, M., Murata, M., Saito, Y., Suzuki, H., Wolff, L. F., & Yoshie, H. (2003) · Journal of Periodontology · doi:10.1902/jop.2003.74.6.849 · Cited by 371
Dallari, D., Savarino, L., Stagni, C., Cenni, E., Cenacchi, A., Fornasari, P. M., Albisinni, U., Rimondi, E., Baldini, N., & Giunti, A. (2007) · The Journal of Bone & Joint Surgery · doi:10.2106/jbjs.f.01026 · Cited by 182
Lee, S., Zheng, Z., Kang, J., Kim, D., Oh, S. H., & Cho, S. B. (2015) · Wound Repair and Regeneration · doi:10.1111/wrr.12250 · Cited by 84
Metadata
- Authors
- Landesberg, R., Roy, M., & Glickman, R. S.
- Venue
- Journal of Oral and Maxillofacial Surgery
- Publisher
- Elsevier BV
- Published
- 2000-03
- Volume
- 58
- Issue
- 3
- Pages
- 297-300
- ISSN
- 0278-2391 (ISSN-L)
- OpenAlex
- W2167417570
- Cited by
- 424
- References
- 1
- FWCI
- 10.32
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Landesberg, R., Roy, M., & Glickman, R. S. (2000). Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. Journal of Oral and Maxillofacial Surgery. https://doi.org/10.1016/s0278-2391(00)90058-2
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
