CYP21 pseudogene transcripts are much less abundant than those from the active gene in normal human adrenocortical cells under various conditions in culture
Abstract
The human steroid 21-hydroxylase pseudogene (CYP21P, also termed CYP21A) is transcribed in the adrenal cortex, but the relative abundance of transcripts from CYP21P and from the active CYP21 gene (also termed CYP21B) is not well established. In the present experiments we cultured primary human adrenocortical cells in defined medium and used RNase protection assays to examine whether there might be a selective increase in the relative abundance of CYP21P transcripts under any of the various regulatory factors known to affect expression of 21-hydroxylase. Differences between the sequences of intron 2 in CYP21P and CYP21 allowed the synthesis of gene-specific probes spanning exon 3 and parts of the adjacent introns. CYP21- and CYP21P-specific probes spanning the site of the start of transcription were also synthesized. CYP21 transcripts were readily detectable. In agreement with previous observations on 21-hydroxylase mRNA and enzyme activity in primary cultures of human adrenocortical cells, the abundance of CYP21 transcripts was increased by cyclic AMP analogues (N6-monobutyryl cyclic AMP and 8-bromo cyclic AMP), insulin, IGF-I and tetradecanoyl phorbol acetate (TPA). However, CYP21P transcripts were not detected in the presence of any of the various regulatory factors known to affect expression of 21-hydroxylase.
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- Authors
- Endoh, A.
- Affiliations
- Baylor College of Medicine (US)
- Corresponding author
- Endoh, A
- ORCID
- Endoh, A
- Venue
- Molecular and Cellular Endocrinology
- Publisher
- Elsevier BV
- Published
- 1998-02-13
- Volume
- 137
- Issue
- 1
- Pages
- 13-19
- ISSN
- 0303-7207 (ISSN-L)
- OpenAlex
- W1981345021
- Cited by
- 13
- References
- 20
- Citations by year
- 2021: 1 · 2017: 1
- Type
- conference abstract
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG-06108)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
- Keywords
- 21-hydroxylase, steroidogenic enzyme gene expression, adrenal cortex, adrenocortical cells, cyclic AMP, insulin, insulin-like growth factor I, phorbol ester, mRNA levels, pseudogene expression
- MeSH terms
- Adrenal Cortex, Cells, Cultured, Enzyme Activation, Gene Expression Regulation, Genes, Humans, Pseudogenes, Steroid 21-Hydroxylase, Transcription, Genetic, Blotting, Northern
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