Comparative catalytic activity of two plant proteinases upon caprine caseins in solution

Proteolysis by cardosin A is faster than by cardosin B.

“proteolysis by cardosin A was reported to be faster than by cardosin B [47].”

The primary and most susceptible sites for attack by both cardosins in caprine β-casein are Leu190-Tyr191 and Leu127-Thr128.

“one concludes that the primary (and most susceptible) sites with regard to attack by both cardosins in caprine β-casein are Leu 190 -Tyr 191 and Leu 127 -Thr 128 (Fig. 1b), whereas in caprine α s1 -casein they are Phe 23 -Val 24 and Phe 153 -Tyr 154 (Fig. 1c).”

Regarding specificity of cardosins A and B upon β-casein, either in caseinate or in pure forms, the major cleavage sites are Leu127-Thr128 and Leu190-Tyr191.

“Regarding the specificity of cardosins A and B upon β-casein, either in caseinate or in pure forms, the major cleavage sites were found to be Leu 127 -Thr 128 and Leu 190 -Tyr 191 ; conversely, in α s -casein, either in caseinate or in pure forms, both cardosins were reported to cleave the peptide bond Phe 153 -Tyr 154 [47].”

The primary and most susceptible sites for attack by both cardosins in caprine αs1-casein are Phe23-Val24 and Phe153-Tyr154.

“one concludes that the primary (and most susceptible) sites with regard to attack by both cardosins in caprine β-casein are Leu 190 -Tyr 191 and Leu 127 -Thr 128 (Fig. 1b), whereas in caprine α s1 -casein they are Phe 23 -Val 24 and Phe 153 -Tyr 154 (Fig. 1c).”

In αs-casein, either in caseinate or in pure forms, both cardosins cleave the peptide bond Phe153-Tyr154.

“Regarding the specificity of cardosins A and B upon β-casein, either in caseinate or in pure forms, the major cleavage sites were found to be Leu 127 -Thr 128 and Leu 190 -Tyr 191 ; conversely, in α s -casein, either in caseinate or in pure forms, both cardosins were reported to cleave the peptide bond Phe 153 -Tyr 154 [47].”

In caprine caseinate, β-caseins are hydrolysed to a higher degree than αs-caseins.

“In caprine caseinate, β-caseins were hydrolysed to a higher degree than α s -caseins, and proteolysis by cardosin A was reported to be faster than by cardosin B [47].”