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Production and isolation of milk-clotting enzyme from Aspergillus versicolor
Abstract
The production of a milk-clotting enzyme by Aspergillus versicolor in 19 different culture media was investigated. Considerable milk-clotting activity was achieved by supplying corn steep liquor with either glucose of maltose. Dephytinization of corn steep liquor had an adverse effect on the production of milk-clotting enzyme. The results indicated that complex organic compounds favoured the production of the enzyme. Precipitation with acetone or tannin was unsuitable, but ammonium sulphate and ethanol above certain concentration produced active fractions.
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“However, additional research has shown that supplementing the growth medium with maltose (Abdel-Fattah and Saleh, 1979) and lactose (Gais et al., 2009) also resulted in the maximum MCA in SSF.”
From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Abdel-Fattah and Saleh, 1979
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Research works printed but not linked: 11
- Abdel-Akher, 1962, Separation of the corn-steeping water proteins by precipitation, Die Stärke, 14, 124, 10.1002/star.19620140404 · DOI
- Abdel-Fattah, 1974, Studies on the production of milk-clotting enzymes, proteolytic enzymes and mucilage by fungi, J. Gen. Microbiol., 84, 327, 10.1099/00221287-84-2-327 · DOI
- Abdel-Fattah, 1972, Distribution pattern of milk-clotting and proteolytic activities in some fungi, Acta Biol. Acad. Sci. Hung., 23, 55
- Abdel-Fattah, 1972, Production and some properties of rennin-like milk-clotting enzyme from Penicillium citrinum, J. Gen. Microbiol., 70, 151, 10.1099/00221287-70-1-151 · DOI
- Bergkvist, 1963, The proteolytic enzymes of Aspergillus oryzae. I. Methods for the estimation and isolation of the proteolytic enzymes, Acta Chem. Scand., 17, 1521, 10.3891/acta.chem.scand.17-1521 · DOI
- Berridge, 1952, Some observations on the determination of the activity of rennet, Analyst, 77, 57, 10.1039/an952770057b · DOI
- Graefe, 1952, Meso-inositol from corn-steep liquor, Die Stärke, 4, 275, 10.1002/star.19520041102 · DOI
- Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
- Osman, 1969, Production of a milk-clotting enzyme preparation by Aspergillus niger and the effect of various factors on its activity, J. Gen. Microbiol., 59, 125, 10.1099/00221287-59-1-125 · DOI
- Srinivasan, 1964, Milk-clotting enzymes from micro-organisms, Appl. Microbiol., 12, 475, 10.1128/AEM.12.6.475-478.1964 · DOI
- Zelinka, 1961, Influence of dephytinization of corn broth on the biosynthesis of penicillin, Biologia, Bratislava, 16, 53
Cited by
Sorted by citations
Milk-clotting enzymes produced by culture of Bacillus subtilis natto.
Shieh, C.-J., Phan Thi, L.-A., & Shih, I.-L. (2009) · Biochemical Engineering Journal · doi:10.1016/j.bej.2008.09.003 · Cited by 99
Sathya, R., Pradeep, B. V., Angayarkanni, J., & Palaniswamy, M. (2009) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-008-0304-0 · Cited by 49
Mamo, J., Kangwa, M., Fernandez-Lahore, H. M., & Assefa, F. (2020) · Brazilian Journal of Microbiology · doi:10.1007/s42770-020-00243-y · Cited by 35
Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review.
Wiśniewski, P., Małkowska-Kowalczyk, M., Łobacz, A., Zadernowska, A., & Żulewska, J. (2025) · Journal of dairy science · doi:10.3168/jds.2025-26973 · Cited by 10
Metadata
- Authors
- Abdel-Fattah, A. F., & Saleh, S. A.
- Affiliations
- National Research Centre (EG)
- ORCID
- Saleh, S.A.
- Venue
- Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite Naturwissenschaftliche Abteilung: Mikrobiologie der Landwirtschaft, der Technologie und des Umweltschutzes
- Publisher
- Elsevier BV
- Published
- 1979-01
- Volume
- 134
- Issue
- 6
- Pages
- 547-550
- ISSN
- 0044-4057 (ISSN-L)
- 0323-6056
- OpenAlex
- W2075305403
- Cited by
- 14
- References
- 0
- Citations by year
- 2020: 1 · 2017: 1 · 2014: 1 · 2013: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Zentralblatt für Mikrobiologie (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- milk-clotting enzyme, Aspergillus versicolor, enzyme production, culture medium optimization, corn steep liquor, glucose, maltose, dephytinization, ammonium sulfate precipitation, ethanol precipitation, tannin precipitation, acetone precipitation, enzyme isolation
- MeSH terms
- Animals, Aspergillus, Cattle, Culture Media, Milk, Chemical Precipitation, Chymosin
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