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Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli
Open accessAbstract
Combination of an origin repair mutagenesis system with a new mutS host strain increased the efficiency of mutagenesis from 46% to 75% mutant clones. Overexpression with the T7 expression system afforded large quantities of proteins from mutant strains. A series of E. coli BE host strains devoid of major outer membrane proteins was constructed, facilitating the purification of mutant porins to homogeneity. This allowed preparation of 149 porin mutants in E. coli used in detailed explorations of the structure and function of this membrane protein to high resolution.
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Cited by
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Robert, V., Volokhina, E. B., Senf, F., Bos, M. P., Gelder, P. V., & Tommassen, J. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040377 · Cited by 326
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Qiao, S., Luo, Q., Zhao, Y., Zhang, X. C., & Huang, Y. (2014) · Nature · doi:10.1038/nature13484 · Cited by 273
Structure of the secretion domain of HxuA from Haemophilus influenzae
Baelen, S., Dewitte, F., Clantin, B., & Villeret, V. (2013) · Acta Crystallographica Section F Structural Biology and Crystallization Communications · doi:10.1107/s174430911302962x · Cited by 44
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Metadata
- Authors
- Prilipov, A.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-06-01
- Volume
- 163
- Issue
- 1
- Pages
- 65-72
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W1986651499
- Cited by
- 234
- References
- 0
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. https://app.cochat.ai/papers/10.1016/s0378-1097(98)00151-7
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- Prilipov, A. (1998). Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. FEMS Microbiology Letters. https://doi.org/10.1016/s0378-1097(98)00151-7
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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