The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing
Open accessAbstract
PCR-ribotying, a typing method based on polymorphism in the 16S-23S intergenic spacer region, has been recently used to investigate outbreaks due to Clostridium difficile. However, this method generates bands of high and close molecular masses which are difficult to separate on agarose gel electrophoresis. To improve reading of banding patterns of PCR-ribotyping applied to C. difficile, a partial sequencing of the rRNA genes (16S and 23S) and intergenic spacer region has been performed, then a new set of primers located closer to the intergenic spacer region has been defined. The new PCR gave reproducible patterns of bands easy to separate on agarose gel electrophoresis. Each of the 10 serogroups and 11 subgroups of serogroup A produced a different pattern. This typing method has evidenced major qualities such as easiness, rapidity and reproducibility. However, its discriminatory power has to be evaluated to validate its importance as a typing tool for C. difficile.
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Cites
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Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping
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TYPING SCHEME FOR CLOSTRIDIUM DIFFICILE: ITS APPLICATION IN CLINICAL AND EPIDEMIOLOGICAL STUDIES
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Cited by
Petrof, E. O., Gloor, G. B., Vanner, S. J., Weese, S. J., Carter, D., Daigneault, M. C., Brown, E. M., Schroeter, K., & Allen-Vercoe, E. (2013) · Microbiome · doi:10.1186/2049-2618-1-3 · Cited by 731
Carey-Ann, B. D., & Carroll, K. C. (2013) · Clinical Microbiology Reviews · doi:10.1128/cmr.00016-13 · Cited by 423
Epidemiology of Recurrences or Reinfections of Clostridium difficile -Associated Diarrhea
Barbut, F., Richard, A., Hamadi, K., Chomette, V., Burghoffer, B., & Petit, J.-C. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.6.2386-2388.2000 · Cited by 355
Clostridium difficile in Retail Ground Meat, Canada
Rodriguez-Palacios, A., Staempfli, H. R., Duffield, T., & Weese, J. S. (2007) · Emerging Infectious Diseases · doi:10.3201/eid1303.060988 · Cited by 254
Kao, D., Wong, K., Franz, R., Cochrane, K., Sherriff, K., Chui, L., Lloyd, C., Roach, B., Bai, A. D., Petrof, E. O., et al. (2021) · The Lancet Gastroenterology & Hepatology · doi:10.1016/s2468-1253(21)00007-8 · Cited by 81
Metadata
- Authors
- Bidet, P.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-06-15
- Volume
- 175
- Issue
- 2
- Pages
- 261-266
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W2077265045
- Cited by
- 398
- References
- 14
- FWCI
- 3.03
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Bidet, P. (1999). Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing. FEMS Microbiology Letters. https://doi.org/10.1016/s0378-1097(99)00204-9
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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