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Cloning and genetic characterization of an evolutionarily conserved human olfactory receptor that is differentially expressed across species
Abstract
We have cloned the full-length cDNA and genomic region of a human prostate specific G-protein coupled receptor with properties characteristic of an olfactory receptor. A partial cDNA sequence of this gene, called PSGR, was recently cloned. The gene contains two exons and one intron of 14.9 kb in its 5'untranslated region, and was mapped to human chromosome 11p15.2. A cluster of transcription initiation sites for the 2.8 kb PSGR mRNA was identified. Cloning of the homologous gene from the mouse revealed 93% amino acid homology between the human and mouse or rat (previously cloned as RA1c) proteins, and 99% identity between the rat and mouse homologs. Although northern analysis indicated expression of the human PSGR homolog was prostate specific, its mRNA could also be detected in the olfactory zone and the medulla oblongata of the human brain. In the mouse, the PSGR gene is predominantly expressed in the brain and colon. In the rat, the PSGR homolog is expressed in the liver in addition to the brain. These data add to the growing body of evidence suggesting that olfactory receptors may have functional roles in tissues other than the olfactory organ, and further, suggest that these functions may vary across species.
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“The only one activated by acetate and propionate is the GPCR olfactory receptor family 51 subfamily member 2 (OR51E2 for humans, olfactory receptor 78 -Olfr78 for mice) (Pluznick et al., 2013), which has been detected in the brain of rodents, but not humans (Yuan et al., 2001).”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Yuan et al., 2001
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Cryan, J. F., O'Riordan, K. J., Cowan, C. S. M., Sandhu, K. V., Bastiaanssen, T. F. S., Boehme, M., Codagnone, M. G., Cussotto, S., Fulling, C., Golubeva, A. V., et al. (2019) · Physiological Reviews · doi:10.1152/physrev.00018.2018 · Cited by 5,286
Metadata
- Authors
- Yuan, T. T.-T., Toy, P., McClary, J. A., Lin, R. J., Miyamoto, N. G., & Kretschmer, P. J.
- Corresponding author
- Kretschmer, Peter J.
- ORCID
- Lin, Richard J.
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 2001-10
- Volume
- 278
- Issue
- 1-2
- Pages
- 41-51
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W2052130505
- Cited by
- 66
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 5 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- olfactory receptors, cDNA cloning, genome mapping, transcription start sites, species-specific expression, medulla oblongata, olfactory epithelium, colon, liver
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 11, Cloning, Molecular, DNA, Gene Expression Regulation, Humans, Male, Molecular Sequence Data, Neoplasm Proteins, RNA, Species Specificity, Tissue Distribution, Blotting, Northern, Sequence Alignment, Conserved Sequence, Sequence Homology, Amino Acid, Sequence Analysis, DNA, Receptors, Odorant, DNA, Complementary, Evolution, Molecular, Transcription Initiation Site, 5' Flanking Region, Mice, Rats
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- Yuan, T. T.-T., Toy, P., McClary, J. A., Lin, R. J., Miyamoto, N. G., & Kretschmer, P. J. (2001). Cloning and genetic characterization of an evolutionarily conserved human olfactory receptor that is differentially expressed across species. Gene. https://doi.org/10.1016/s0378-1119(01)00709-0
