Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization
Abstract
The ADAM (A Disintegrin And Metalloprotease) family is known to have important roles in various developmental systems, e.g., myogenesis and neurogenesis. In this study, we searched for ADAMs that may function in spermatogenesis or fertilization, and have cloned and sequenced four new mouse ADAM cDNAs: ADAM 24, ADAM 25, ADAM 26 and ADAM 27. The deduced amino acid sequences show that all four contain the complete domain organization common to ADAM family members. Messenger RNA for each of the four ADAMs was found only in the testis. The conserved zinc-dependent metalloprotease active site HEXGHXXGXXHD was found in the metalloprotease domain of three of the novel ADAMs, suggesting that they are testis-specific proteases, to which we give the alternative names: testase 1, ADAM 24; testase 2, ADAM 25; and testase 3, ADAM 26. Using RNA extracted from testes of pre-pubertal males of increasing age (8-40days), we found that adult levels of transcription, assessed in Northern blots, are reached by day 20 (ADAM 27), day 25 (ADAMs 24 and 25) and in the range day 25-50 (ADAM 26). These results suggest that each ADAM is transcribed in spermatogenic cells in a regulated pattern at a specific developmental stage.
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Several proteins in spermatogenesis seem to carry two HHMCs: SSFA2, SPAG17, ADAM18 and WDR52, out of which ADAM18 and SPAG17 also carry AHMCs.
“we find that several proteins in spermatogenesis seem to carry two HHMCs: Sperm Specific Antigen 2 (SSFA2), Sperm Associated Antigen 17 (SPAG17), ADAM18172 and WDR52173, out of which ADAM18 and SPAG17 also carry AHMCs.”
From A catalog of single nucleotide changes distinguishing modern humans from archaic hominins (2019) · cites this paper as [172]
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A catalog of single nucleotide changes distinguishing modern humans from archaic hominins
Kuhlwilm, M., & Boeckx, C. (2019) · Scientific Reports · doi:10.1038/s41598-019-44877-x · Cited by 108 · see citing works
- Authors
- Zhu, G.-Z., Lin, Y., Myles, D. G., & Primakoff, P.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Zhu, Guo-Zhang
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 1999-07
- Volume
- 234
- Issue
- 2
- Pages
- 227-237
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W1997466276
- Cited by
- 45
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2019: 1 · 2015: 1 · 2013: 1 · 2012: 3
- FWCI
- 4.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- U.S. Public Health Service (U54 29125)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Keywords
- ADAM family, spermatogenesis, fertilisation (biology), zinc-dependent metalloprotease, gene expression regulation, mice
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Fertilization, Male, Membrane Glycoproteins, Metalloendopeptidases, Molecular Sequence Data, Phylogeny, RNA, Messenger, Spermatogenesis, Testis, Tissue Distribution, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Analysis, DNA, DNA, Complementary, Gene Expression Regulation, Developmental, Evolution, Molecular, Disintegrins, Mice, ADAM Proteins
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