Cited by 77Bone Metabolism and DiseasesBone Tissue Engineering MaterialsBone fractures and treatments

Enhancement of bone defect healing in old rats by TGF-β and IGF-1

Blumenfeld, I., Srouji, S., Lanir, Y., Laufer, D., Livne, E.·2002·Experimental Gerontology

Abstract

Bone defects are often created in order to repair bone pathologies. In the aging population, the healing of such defects is very limited. Bone healing in aging depends on the availability of various hormone and growth factors. The ability of growth factors to enhance bone formation in femoral defects in old rats was tested. Bone defects were induced in femurs of old rats. A single dose of transforming growth factor-beta (TGF-beta), IGF-1, TGF-beta+IGF-1 or saline was inserted in the defect and bones were tested after 2 and 4 weeks. Radiology revealed that mineralization appeared in the 2 weeks group in defects treated with TGF-beta and in defects treated with TGF-beta, TGF-beta+IGF-1 in the 4 weeks groups. Computerized tomography (CT) coronal and axial images revealed that 4 weeks after treatment with TGF-beta+IGF-1, a complete bone bridge was observed. Morphology revealed that these defects were filled with trabecular bone. A less pronounced bone healing was observed after TGF-beta or IGF-1, while control specimens revealed partial healing of the bone defect. Biomechanical tests indicated that treatment with TGF-beta, IGF-1 or TGF-beta+IGF-1 resulted in a significant increase of bone bending rigidity compared to control in the 4 weeks group and that TGF-beta+IGF-1 was the most inductive in this respect. The ability to induce bone healing in aging by TGF-beta+IGF-1 is of a great clinical importance for restoration of bone strength and biomechanical properties of bone defects in aging.

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Crossref · 10.1016/s0531-5565(01)00215-7

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Authors
Blumenfeld, I., Srouji, S., Lanir, Y., Laufer, D., & Livne, E.
Affiliations
  • Rambam Health Care Campus (IL)
  • Technion – Israel Institute of Technology (IL)
Corresponding author
Livne, E.
Venue
Experimental Gerontology
Publisher
Elsevier BV
Published
2002-04
Volume
37
Issue
4
Pages
553-565
ISSN
0531-5565 (ISSN-L)
OpenAlex
W2072531220
Cited by
77
References
32
Citations by year
2024: 1 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 7 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
FWCI
2.19
Type
journal article
Language
en
Open access
closed
Locations
  • Experimental Gerontology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Epidemiology › Bone fractures and treatments
Keywords
bone defect repair, aging, TGF-beta, insulin-like growth factor I, femoral defect, bone mineralization, bone bridge formation, trabecular bone, bone mechanical properties, growth factors, aged rats
MeSH terms
Aging, Animals, Biomechanical Phenomena, Female, Insulin-Like Growth Factor I, Stress, Mechanical, Transforming Growth Factor beta, Bone Remodeling, Fracture Healing, Rats, Wistar, Rats

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