A simple LC method with UV detection for the analysis of creatine and creatinine and its application to several creatine formulations
Abstract
The objective of this study was to develop a simple and sensitive LC method for the determination of creatine and creatinine in various creatine supplement formulations. The chromatographic system comprised of a LC-600 pump, SCL-6B system controller, and SPD-6AV detector (Shimadzu, Japan). The mobile phase consisted of 0.045 M ammonium sulfate in water. The chromatographic separation was achieved at ambient temperature on a Betabasic C-18 column (250 x 4.6 mm, Keystone Sci.). The flow rate was maintained at 0.75 ml/min and effluents are monitored at 205 nm. 4-(2-Aminoethyl)benzene sulfonamide was used as an internal standard (IS). This method required less than 7 min of chromatographic time. The standard curves were linear over the concentration range of 1-100 microg/ml for creatine and 2-100 microg/ml for creatinine, respectively. The relative standard deviations (RSD) for the within-day and day-to-day precision for creatine were within 1.0-4.6 and 2.2-4.7%, respectively. The RSD for the accuracy of creatine assay was in the range of 2.4-4.7%. The RSD values for the within-day precision, day-to-day precision and accuracy for creatinine validation were 1.7-4.4, 2.3-5.4 and 2.4-4.8%, respectively. This method was used to determine: (i) the creatine concentration in various marketed products; (ii) saturated solubility of various creatine salts; and (iii) stability of creatine in aqueous solution. In conclusion, a simple and sensitive LC method with UV detection was developed fo…
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The HPLC method developed earlier was found to be suitable to determine all 3 compounds (creatine, creatinine, citric acid).
“The HPLC method developed earlier was found to be suit-able to determine all 3 compounds.11”
From Evaluation of the stability of creatine in solution prepared from effervescent creatine formulations (2003) · cites this paper as [11]
The linearity ranges for creatine and creatinine along with all the validation parameters have been published elsewhere.
“The linearity ranges for creatine and creatinine along with all the validation parameters have been published elsewhere.11”
From Evaluation of the stability of creatine in solution prepared from effervescent creatine formulations (2003) · cites this paper as [11]
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Safety of Creatine Supplementation
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Evaluation of the stability of creatine in solution prepared from effervescent creatine formulations
Ganguly, S., Jayappa, S., & Dash, A. K. (2003) · AAPS PharmSciTech · doi:10.1208/pt040225 · Cited by 20 · see citing works
Self-citationEffect of water activity and temperature on the stability of creatine during storage
Uzzan, M., Nechrebeki, J., Zhou, P., & Labuza, T. P. (2009) · Drug Development and Industrial Pharmacy · doi:10.1080/03639040902755197 · Cited by 11 · see citing works
- Authors
- Dash, A. K., & Sawhney, A.
- Affiliations
- Creighton University (US)
- Corresponding author
- Dash, Alekha K
- ORCID
- Dash, Alekha K
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2002-07
- Volume
- 29
- Issue
- 5
- Pages
- 939-945
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W1977360630
- Cited by
- 58
- References
- 1
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 3 · 2020: 4 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- creatine, creatinine, creatine monohydrate, UV detection, internal standard
- MeSH terms
- Chromatography, High Pressure Liquid, Creatine, Creatinine, Sensitivity and Specificity, Spectrophotometry, Ultraviolet, Reproducibility of Results
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