Cited by 192Blood properties and coagulationRedox biology and oxidative stressErythrocyte Function and Pathophysiology

Effect of Superoxide Anions on Red Blood Cell Rheologic Properties

Baskurt, Oguz K., Temiz, Aysegul, Meiselman, Herbert J.·1998·Free Radical Biology and Medicine

Abstract

The human red blood cell (RBC) is known to be susceptible to oxidant damage, with both structural and functional properties altered consequent to oxidant attack. Such oxidant-related alterations may lead to changes of RBC rheologic behavior (i.e., deformability, aggregability). Two different models of oxidant stress were used in this study to generate superoxide anions either internal or external to the RBC. Our results indicate that generation of superoxide within the RBC by phenazine methosulfate decreases RBC deformability without effects on cell aggregation. Conversely, superoxide generated externally by the xanthine oxidase-hypoxanthine system primarily affects RBC aggregability: the shear rate necessary to disaggregate RBC was markedly increased while the extent of aggregation decreased slightly. Increased disaggregation shear rate (i.e., greater aggregate strength) as a result of superoxide radical damage may adversely affect the dynamics of blood flow in low-shear portions of the circulation, and may also play a role in the no-reflow phenomena encountered after ischemia-reperfusion.

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Crossref · 10.1016/s0891-5849(97)00169-x

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Authors
Baskurt, O. K., Temiz, A., & Meiselman, H. J.
Affiliations
University of Southern California (US)
Corresponding author
Meiselman, Herbert J.
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
1998-01
Volume
24
Issue
1
Pages
102-110
ISSN
0891-5849 (ISSN-L)
OpenAlex
W2052023508
Cited by
192
References
51
Citations by year
2026: 3 · 2025: 6 · 2024: 3 · 2023: 6 · 2022: 10 · 2021: 4 · 2020: 5 · 2019: 5 · 2018: 16 · 2017: 9 · 2016: 6 · 2015: 9 · 2014: 13 · 2013: 8 · 2012: 12
FWCI
5.00
Type
journal article
Language
en
Open access
closed
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p60hl048484-10)
  • National Heart, Lung, and Blood Institute (HL48484, HL 15722)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Erythrocyte Function and Pathophysiology
Keywords
superoxide, red blood cell rheology, erythrocyte deformability, erythrocyte aggregation, oxidative damage, phenazine methosulfate, no-reflow phenomenon, ischemia-reperfusion
MeSH terms
Erythrocyte Aggregation, Erythrocyte Deformability, Free Radicals, Humans, Methylphenazonium Methosulfate, Reference Values, Stress, Mechanical, Superoxides, Xanthine Oxidase, Reactive Oxygen Species, Oxidative Stress

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