The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Pilocarpine-induced status epilepticus results in mossy fiber sprouting and spontaneous seizures in C57BL/6 and CD-1 mice
Abstract
Several rodent models are available to study the cellular mechanisms associated with the development of temporal lobe epilepsy (TLE), but few have been successfully transferred to inbred mouse strains commonly used in genetic mutation studies. We examined spontaneous seizure development and correlative axon sprouting in the dentate gyrus of CD-1 and C57BL/6 mice after systemic injection of pilocarpine. Pilocarpine induced seizures and status epilepticus (SE) after systemic injection in both strains, although SE onset latency was greater for C57BL/6 mice. There were also animals of both strains which did not experience SE after pilocarpine treatment. After a period of normal behavior for several days after the pilocarpine treatment, spontaneous tonic-clonic seizures were observed in most CD-1 mice and all C57BL/6 that survived pilocarpine-induced SE. Robust mossy fiber sprouting into the inner molecular layer was observed after 4-8 weeks in mice from both strains which had experienced SE, and cell loss was apparent in the hippocampus. Mossy fiber sprouting and spontaneous seizures were not observed in mice that did not experience a period of SE. These results indicate that pilocarpine induces spontaneous seizures and mossy fiber sprouting in both CD-1 and C57BL/6 mouse strains. Unlike systemic kainic acid treatment, the pilocarpine model offers a potentially useful tool for studying TLE development in genetically modified mice raised on the C57BL/6 background.
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“At seven days after SE and during periods of immobility in freely-moving mice, although convulsive seizures were not observed (Racine’s score > 3), consistent with previous observations [34, 35] the dorsal hippocampus displayed disruptions in electrical patterns, mainly in all hippocampal layers (Fig. 2A).”
From Hippocampal neuro-networks and dendritic spine perturbations in epileptogenesis are attenuated by neuroprotectin D1 (2015) · cites this paper as [35]
In the citing paper
“Since chronic recurrent seizures are a consequence of pilocarpine-induced SE in all adult mice [27, 34, 35]”
From Hippocampal neuro-networks and dendritic spine perturbations in epileptogenesis are attenuated by neuroprotectin D1 (2015) · cites this paper as [35]
In the citing paper
“The pilocarpine model of TLE in adult mice shows an onset of recurrent spontaneous seizures 7 days after SE that can continue for at least 7 weeks [27, 34, 35].”
From Hippocampal neuro-networks and dendritic spine perturbations in epileptogenesis are attenuated by neuroprotectin D1 (2015) · cites this paper as [35]
Citations
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Evidence of functional mossy fiber sprouting in hippocampal formation of kainic acid-treated rats
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Jiang, X., Wu, W., Yang, Z., & Meng, C. (2026) · Journal of Integrative Neuroscience · doi:10.31083/jin54266
Metadata
- Authors
- Shibley, H., & Smith, B. N.
- Affiliations
- Tulane University (US)
- Corresponding author
- Smith, Bret N.
- ORCID
- Smith, Bret N.
- Venue
- Epilepsy Research
- Publisher
- Elsevier BV
- Published
- 2002-04
- Volume
- 49
- Issue
- 2
- Pages
- 109-120
- ISSN
- 0920-1211 (ISSN-L)
- OpenAlex
- W2042575144
- Cited by
- 201
- References
- 32
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 3 · 2023: 5 · 2022: 4 · 2021: 11 · 2020: 12 · 2019: 12 · 2018: 10 · 2017: 8 · 2016: 12 · 2015: 10 · 2014: 9 · 2013: 15 · 2012: 15
- FWCI
- 2.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Epilepsy Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Louisiana Board of Regents
- Tulane University
- Topics
- Health Sciences › Medicine › Clinical Neurology › Epilepsy research and treatment
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Pharmacological Effects and Toxicity Studies
- Keywords
- pilocarpine, status epilepticus, mossy fiber sprouting, temporal lobe epilepsy, spontaneous seizures, dentate gyrus, hippocampal neuronal loss, C57BL/6 mice, ICR mice, mouse strain differences, kainic acid model, inbred mouse models
- MeSH terms
- Animals, Convulsants, Male, Mice, Inbred C57BL, Mice, Inbred Strains, Neurons, Pilocarpine, Seizures, Status Epilepticus, Cell Death, Dentate Gyrus, Mossy Fibers, Hippocampal, Mice
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