Osr2, a new mouse gene related to Drosophila odd-skipped, exhibits dynamic expression patterns during craniofacial, limb, and kidney development
Open accessAbstract
We have isolated a new mouse gene, odd-skipped related 2 (Osr2), that encodes a zinc finger containing protein related to Drosophila Odd-skipped. The putative OSR2 protein shares 65% amino acid sequence identity overall and 98% sequence identity in the zinc finger region, respectively, with the previously reported Osr1 gene product. During mouse embryonic development, Osr2 expression is first detected at E9.25, specifically in the mesonephric vesicles. By E10.0, Osr2 expression is also observed in the rostro-lateral mandibular mesenchyme immediately adjacent to the maxillary processes. In the developing limb buds, Osr2 is expressed in a unique mesenchymal domain and the onset of Osr2 expression follows a distinct dorsal to ventral developmental time sequence beginning in the forelimb and then in the hindlimb. Osr2 exhibits a dynamic expression pattern during craniofacial development, in the mandibular and maxillary processes as well as the developing palate. Osr2 is also expressed at sites of epithelial-mesenchymal interactions during tooth and kidney development.
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HIC1, HIC2, GZF1, OSR1, OSR2, and SALL4 have all been implicated in development.
“HIC1, HIC2, GZF1, OSR1, OSR2, and SALL4 have all been implicated in development”
From Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN (2018) · cites this paper as [28]
The Results and Figure 2 describe OSR1 and OSR2 expression in developing mouse tissues, supporting their association with development. The paper does not address HIC1, HIC2, GZF1, or SALL4, so it does not support the full list. (LLM, conf=0.88)
Experimental — accuracy not yet measuredHIC1, HIC2, GZF1, OSR1, OSR2, and SALL4 could contribute to developmental abnormalities in fetuses exposed to thalidomide.
“and could contribute to developmental abnormalities in fetuses exposed to thalidomide (25–30).”
From Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN (2018) · cites this paper as [28]
The Results and in situ hybridization passages (page unavailable) show Osr2 expression in developing mouse limbs and craniofacial tissues, providing developmental context for one gene in the claim. They do not link Osr2 to thalidomide exposure or fetal abnormalities, and this paper does not address HIC1, HIC2, GZF1, or SALL4. (LLM, conf=0.78)
Experimental — accuracy not yet measuredSeveral ZFs identified as degraded in functional experiments, or in vitro binders that could be targeted with further drug development, have been implicated in human disease.
“Several of the ZFs identified as degraded in functional experiments, or in vitro binders that could be targeted with further drug development, have been implicated in human disease.”
From Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN (2018) · cites this paper as [28]
The Results section identifies mouse OSR2 as a zinc-finger protein, and §2.1 identifies a putative human OSR2 gene. Neither passage links any zinc-finger protein to human disease; searches for disease implications, experimental degradation, in vitro binding, and drug development found no such discussion in our copy. Page numbers were not available. (LLM, conf=0.76)
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Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN
Sievers, Q. L., Petzold, G., Bunker, R. D., Renneville, A., Słabicki, M., Liddicoat, B. J., Abdulrahman, W., Mikkelsen, T., Ebert, B. L., & Thomä, N. H. (2018) · Science · doi:10.1126/science.aat0572 · Cited by 537 · see citing works
- Authors
- Lan, Y., Kingsley, P. D., Cho, E.-S., & Jiang, R.
- Affiliations
- University of Rochester Medicine (US)
- Corresponding author
- Jiang, Rulang
- Venue
- Mechanisms of Development
- Publisher
- Elsevier BV
- Published
- 2001-09
- Volume
- 107
- Issue
- 1-2
- Pages
- 175-179
- ISSN
- 0925-4773 (ISSN-L)
- OpenAlex
- W2034717801
- Cited by
- 108
- References
- 11
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 5 · 2023: 1 · 2022: 8 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 8 · 2016: 7 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 5
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Mechanisms of Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Dental and Craniofacial Research (R01DE013681, DE13681)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Cleft Lip and Palate Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Keywords
- Osr2, zinc finger proteins, OSR1, mouse embryo development, craniofacial development, limb bud development, kidney development, palate development, epithelial-mesenchymal interactions, tooth development, gene expression patterns
- MeSH terms
- Amino Acid Sequence, Animals, Extremities, Embryonic and Fetal Development, Jaw, Kidney, Molecular Sequence Data, Skull, Transcription Factors, Gene Expression, In Situ Hybridization, Gene Expression Profiling, Mice
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