Fruit juice consumption modulates antioxidative status, immune status and DNA damage
Abstract
Polyphenolic compounds exert a variety of physiological effects in vitro including antioxidative, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men on a low-polyphenol diet, we determined the effects of 2 polyphenol-rich juices (330 ml/d) supplemented for 2 weeks on bioavailability of polyphenols, markers of antioxidative and immune status, and reduction of DNA damage. Juices provided 236 mg (A) and 226 mg (B) polyphenols with cyanidin glycosides (A) and epigallocatechin gallate (B) as major polyphenolic ingredients. There was no accumulation of plasma polyphenols after two weeks of juice supplementation. In contrast, plasma malondialdehyde decreased with time during juice interventions. Moreover, juice consumption also increased lymphocyte proliferative responsiveness, with no difference between the two juices. Interleukin-2 secretion by activated lymphocytes and the lytic activity of natural killer cells were significantly increased by both juices. Juice intervention had no effect on single DNA strand breaks, but significantly reduced oxidative DNA damage in lymphocytes. A time-delay was observed between the intake of fruit juice and the reduction of oxidative DNA damage and the increase in interleukin-2 secretion. We conclude that consumption of either juice enhanced antioxidant status, reduced oxidative DNA damage and stimulated immune cell functions. However, fruit juice consumption for 2 weeks did not result in elevated plasma polyphenols in subjects after overnight fasting. Further studies should focus on the time-delay between juice intake and changes in measured physiological functions, as well as on active polyphenolic metabolites mediating the observed effects.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Benefits of berry intervention were seen in healthy subjects and in those with existing metabolic risk factors.
“Benefits were seen in healthy subjects and in those with existing metabolic risk factors.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [40]
Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (fresh, juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.
“Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (either fresh, or as juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [40]
The most significant outcomes of clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.
“The most significant outcomes of these clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [40]
Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.
“Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [40]
The citation lists haven’t been fetched yet
References 62 · Cited by 233
- Authors
- Bub, A., Watzl, B., Blockhaus, M., Briviba, K., Liegibel, U., Müller, H., Pool-Zobel, B. L., & Rechkemmer, G.
- Affiliations
- Heidelberg University (DE)
- Friedrich Schiller University Jena (DE)
- Venue
- The Journal of Nutritional Biochemistry
- Publisher
- Elsevier BV
- Published
- 2003-02
- Volume
- 14
- Issue
- 2
- Pages
- 90-98
- ISSN
- 0955-2863 (ISSN-L)
- OpenAlex
- W1978369972
- Cited by
- 233
- References
- 62
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 8 · 2021: 8 · 2020: 9 · 2019: 11 · 2018: 9 · 2017: 12 · 2016: 6 · 2015: 11 · 2014: 15 · 2013: 18 · 2012: 12
- FWCI
- 7.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Nutritional Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- OpenAgrar (submittedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- fruit juice consumption, polyphenols, cyanidin glycosides, epigallocatechin gallate, antioxidant status, oxidative DNA damage, immune status, lymphocyte proliferation, interleukin-2, NK cell cytotoxicity, malondialdehyde, polyphenol bioavailability, single-strand breaks
- MeSH terms
- Adult, Antioxidants, Beverages, Biological Availability, Carotenoids, DNA Damage, Flavonoids, Fruit, Humans, Immunity, Interleukin-2, Lymphocyte Activation, Male, Malondialdehyde, Oxidation-Reduction, Phenols, Polymers, Biomarkers, Cross-Over Studies, Polyphenols
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
