Applications of yeast in biotechnology: protein production and genetic analysis
Abstract
Improvements in yeast expression systems, coupled with the development of yeast surface display and refinements in two-hybrid methodology, are expanding the role of yeasts in the process of understanding and engineering eukaryotic proteins.
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S. cerevisiae, traditionally considered non-pathogenic, has been reported as an opportunistic pathogen in immunocompromised patients.
“Numerous cases of superficial or mild systemic infections caused by Saccharomyces cerevisiae, a yeast”
Yeast isolates currently used in industrial or biotechnological processes may be potentially pathogenic.
“From another point of view, yeasts isolates currently in use for industrial or biotechnological processes may be potentially pathogenic.20,26,34”
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“The methylotrophic yeast Pichia pastoris is an eYcient host for expression and secretion of heterologous proteins [2, 10 ].”
Plant‐based biopharming of recombinant human lactoferrin
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“…gene expression under the strong regulated alcohol oxidase I (AOX1) promoter, marker/host strain combination and also the high cell culture density, make P. pastoris an efficient bioreactor for recombinant human Lf production (Cereghino and Cregg, 1999; Kruzel and Zimecki, 2002; Jo et al., 2011).”
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“Cultures can reach cell densities up to 150 g DCW L−1 in the controlled environment of a bioreactor (Cereghino & Cregg, 1999, 2000).”
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“An inducible expression system is particularly important because the strongly regulated promoter (AOX 1) is induced by this promoter [19].”
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“It has also the ability to perform eukaryotic modifications and appropriate folding with the advantage of the easy manipulation and the rapid growth usual in prokaryotic cells [9].”
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Rodrigues, V. D. M., Lopes, D. S., Castanheira, L. E., Gimenes, S. N. C., de Souza, D. L. N., Achê, D. C., Borges, I. P., Yoneyama, K. A. G., & Rodrigues, R. S. (2015) · Current Topics in Medicinal Chemistry · doi:10.2174/1568026615666150217120347 · Cited by 29 · see citing works
“Among the most studied systems, heterologous expression in Pichia pastoris has been used as a molecular biology tool due to its ability to perform posttranslational modifications [156], a characteristic important to enzymatic, biological and clinical activities.”
BackgroundYu, P., Zhu, Q., Chen, K., & Lv, X. (2014) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-014-1292-5 · Cited by 29 · see citing works
“been a major strategy for the genetic improvement of endoplasmic reticulum resident proteins [25, 32–34].”
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“Furthermore, there is a fire hazard using methanol as an inducer and it is inappropriate for the production of foods and drugs (Cereghino and Cregg, 1999).”
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An improved system for estradiol-dependent regulation of gene expression in yeast
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Plasmodium Permeomics: Membrane Transport Proteins in the Malaria Parasite
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“The high levels of expression obtained from most standard yeast expression systems may overload the secretory pathway, reducing plasma membrane expression [ 15 , 44].”
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“Experiments were carried out in triplicate for the establishment of standard curves and in duplicate for clone analysis with the following program: 95 °C for 10 min as an initial denaturation, 40 cycles of 95 °C for 15 s, and 60 °C for 1 min.”
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Saccharomyces cerevisiae Ghosts Using the Sponge-Like Re-Reduced Protocol
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Heterologous Expression of Biologically Active Porcine Lactoferrin in Pichia Pastoris Yeast
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“…GAP promoter provides a constitutive expression level, the regulatable AOX 1 promoter “switches off” foreign protein expression before induction, minimizing the selection of nonexpressing transformants during the growth phase and allowing tighter control of expression (Cereghino and Cregg, 1999).”
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- Authors
- Cereghino, G. P. L., & Cregg, J. M.
- Corresponding author
- Cregg, James M
- Venue
- Current Opinion in Biotechnology
- Publisher
- Elsevier BV
- Published
- 1999-10
- Volume
- 10
- Issue
- 5
- Pages
- 422-427
- ISSN
- 0958-1669 (ISSN-L)
- OpenAlex
- W2124157676
- Cited by
- 176
- References
- 41
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 7 · 2022: 3 · 2021: 5 · 2020: 8 · 2019: 3 · 2018: 6 · 2017: 5 · 2016: 8 · 2015: 3 · 2014: 9 · 2013: 9 · 2012: 10
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (7r01dk043698-09)
- U.S. Public Health Service (ER20334)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-43698)
- National Institute of Environmental Health Sciences (ES05759, F32ES005759)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- yeast, protein expression systems, yeast surface display, two-hybrid system, genetic analysis, recombinant protein expression
- MeSH terms
- Biotechnology, Pichia, Recombinant Proteins, Saccharomyces cerevisiae, Yeasts, Protein Engineering, Gene Expression Regulation, Fungal
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